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dc.contributor.authorGiraddi, Rajshekhar R
dc.contributor.authorShehata, Mona
dc.contributor.authorGallardo, Mercedes
dc.contributor.authorBlasco, MA 
dc.contributor.authorSimons, Benjamin D
dc.contributor.authorStingl, John
dc.date.accessioned2020-05-08T15:12:15Z
dc.date.available2020-05-08T15:12:15Z
dc.date.issued2015-10-29
dc.identifier.citationNat Commun. 2015;6:8487.es_ES
dc.identifier.issn2041-1723es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10001
dc.description.abstractThe cycling properties of mammary stem and progenitor cells is not well understood. To determine the division properties of these cells, we administered synthetic nucleosides for varying periods of time to mice at different stages of postnatal development and monitored the rate of uptake of these nucleosides in the different mammary cell compartments. Here we show that most cell division in the adult virgin gland is restricted to the oestrogen receptor-expressing luminal cell lineage. Our data also demonstrate that the oestrogen receptor-expressing, milk and basal cell subpopulations have telomere lengths and cell division kinetics that are not compatible with these cells being hierarchically organized; instead, our data indicate that in the adult homeostatic gland, each cell type is largely maintained by its own restricted progenitors. We also observe that transplantable stem cells are largely quiescent during oestrus, but are cycling during dioestrus when progesterone levels are high.es_ES
dc.description.sponsorshipWe thank the members of Stingl lab, Doug Winton, Jason Carroll, Robert Clarke, Phil Jones and Hamid Raza Ali for scientific discussions. We thank the core facilities at the Cancer Research UK-CI for enabling experiments. In particular, Loic Tauzin, Nina Lane and Mateuz Strzelecki for assistance with cell sorting; the Biological Resources Unit for animal husbandry; and Histopathology staff, in particular Leigh-Anne McDuffus and Cara Walters. J. Stingl's laboratory acknowledges the support of The University of Cambridge, Cancer Research UK (core grant number C14303/A17197) and Hutchison Whampoa Limited. M.A. Blasco's laboratory is funded by the Spanish Ministry of Economy and Competitiveness Project SAF2013-45111RETOS, the European Union FP7 Project EUROBATS, the European Research Council (ERC) Project TEL STEM CELL (GA#232854), the Regional Government of Madrid project 2 + 2 ReCaRe, the AXA Research Fund and the Fundacion Botin.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Group es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshFemale es_ES
dc.subject.meshKinetics es_ES
dc.subject.meshMammary Glands, Animal es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshReceptors, Estrogenes_ES
dc.subject.meshStem Cells es_ES
dc.subject.meshCell Self Renewal es_ES
dc.titleStem and progenitor cell division kinetics during postnatal mouse mammary gland developmentes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID26511661es_ES
dc.format.volume6es_ES
dc.format.number1es_ES
dc.format.page8487es_ES
dc.identifier.doi10.1038/ncomms9487es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderUnión Europea 
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.contributor.funderFundación AXA 
dc.contributor.funderBotín Foundation 
dc.contributor.funderHutchison Whampoa Limited 
dc.contributor.funderCancer Research UK (Reino Unido) 
dc.contributor.funderComunidad de Madrid (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn2041-1723es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/ncomms9487.es_ES
dc.identifier.journalNature communicationses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Telómeros y Telomerasaes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF2013-45111RETOSes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/EC/FP7/232854es_ES
dc.rights.accessRightsopen accesses_ES


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