Publication: A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus
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Diaz-Guerra, Teresa M. ISCIII 
Mellado, Emilia ISCIII 
Cuenca-Estrella, Manuel ISCIII
Rodriguez-Tudela, Juan Luis ISCIII
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Abstract
The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.
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Erratum in: Antimicrob Agents Chemother. 2004 Mar;48(3):1071.
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Amino Acid Substitution Antifungal Agents Aspergillus fumigatus Base Sequence Cytochrome P-450 Enzyme System Drug Resistance, Fungal Electroporation Fungal Proteins Itraconazole Microbial Sensitivity Tests Molecular Sequence Data Point Mutation Reverse Transcriptase Polymerase Chain Reaction Transformation, Genetic
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Antimicrob Agents Chemother. 2003 Mar;47(3):1120-4.