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A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus

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dc.contributor.authorDiaz-Guerra, Teresa M.
dc.contributor.authorMellado, Emilia
dc.contributor.authorCuenca-Estrella, Manuel
dc.contributor.authorRodriguez-Tudela, Juan Luis
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)
dc.date.accessioned2019-08-13T12:05:35Z
dc.date.available2019-08-13T12:05:35Z
dc.date.issued2003-03
dc.descriptionErratum in: Antimicrob Agents Chemother. 2004 Mar;48(3):1071.
dc.description.abstractThe genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.es_ES
dc.description.sponsorshipThis work was supported in part by grants MPY1078/99 and MPY1281/01 from Instituto de Salud Carlos III. E.M. held a contract, Ramón y Cajal, from the Ministry of Science and Technology. T.M.D.-G. is a postdoctoral fellow of the Instituto de Salud Carlos III (grant 99/4149). We thank D. W. Denning and J. Mosquera, University of Manchester (Manchester, United Kingdom) for providing strains AF-72, AF-90, AF-91, AF-1422, F/6929, F/7075, Br130, Br181, and SO/3829. We also thank E. Dannaoui, Universite Claude Bernard Lyon I (Lyon, France) for the gift of strains AF-1119 and AF-1237. We are grateful to Daniel Lopez, Instituto de Salud Carlos III (Madrid, Spain), for critical reading of the manuscript. We thank Gema del Rio for invaluable technical assistance.es_ES
dc.format.number3es_ES
dc.format.page1120-4es_ES
dc.format.volume47es_ES
dc.identifier.citationAntimicrob Agents Chemother. 2003 Mar;47(3):1120-4.es_ES
dc.identifier.doi10.1128/aac.47.3.1120-1124.2003es_ES
dc.identifier.issn0066-4804es_ES
dc.identifier.journalAntimicrobial agents and chemotherapyes_ES
dc.identifier.pubmedID12604551es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8234
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiology (ASM)
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MPY1078/99es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MPY1281/01es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/99/4149es_ES
dc.relation.publisherversionhttps://doi.org/10.1128/AAC.47.3.1120-1124.2003es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Substitutiones_ES
dc.subject.meshAntifungal Agentses_ES
dc.subject.meshAspergillus fumigatuses_ES
dc.subject.meshBase Sequencees_ES
dc.subject.meshCytochrome P-450 Enzyme Systemes_ES
dc.subject.meshDrug Resistance, Fungales_ES
dc.subject.meshElectroporationes_ES
dc.subject.meshFungal Proteinses_ES
dc.subject.meshItraconazolees_ES
dc.subject.meshMicrobial Sensitivity Testses_ES
dc.subject.meshMolecular Sequence Dataes_ES
dc.subject.meshPoint Mutationes_ES
dc.subject.meshReverse Transcriptase Polymerase Chain Reactiones_ES
dc.subject.meshTransformation, Genetices_ES
dc.titleA point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatuses_ES
dc.typejournal articlees_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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