Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/19312
Título
A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos.
Autor(es)
Gimenez-Llorente, Daniel | Portela, Marta | Losada, Ana CNIO | Manzanares, Miguel CNIC | Andreu, Maria Jose CNIC
Fecha de publicación
2023-04-30
Cita
STAR Protoc. 2023 Apr 30;4(2):102267.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Hi-C studies the three-dimensional structure of the genome by detecting genome-wide chromatin regions that are in spatial proximity within the nucleus. We developed single-blastocyst Hi-C in mutant mouse embryos to genotype them on sequence. We describe steps for embryo fixation and nuclei permeabilization, after which chromatin is digested and re-ligated having incorporated a biotin-labeled nucleotide at the ligation junction. After cross-link reversal, we then detail purification of immobilized chimeric DNA ligations, library generation, sequencing, and genome-wide analysis of interactions. For complete details on the use and execution of this protocol, please refer to Andreu et al. (2022).1.
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- Nombre:
- A protocol to study three_dime ...
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- 4.350Mb
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- Artículo