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A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos.

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dc.contributor.authorGimenez-Llorente, Daniel
dc.contributor.authorPortela, Marta
dc.contributor.authorLosada, Ana
dc.contributor.authorManzanares, Miguel
dc.contributor.authorAndreu, Maria Jose
dc.date.accessioned2024-05-09T08:48:22Z
dc.date.available2024-05-09T08:48:22Z
dc.date.issued2023-04-30
dc.description.abstractHi-C studies the three-dimensional structure of the genome by detecting genome-wide chromatin regions that are in spatial proximity within the nucleus. We developed single-blastocyst Hi-C in mutant mouse embryos to genotype them on sequence. We describe steps for embryo fixation and nuclei permeabilization, after which chromatin is digested and re-ligated having incorporated a biotin-labeled nucleotide at the ligation junction. After cross-link reversal, we then detail purification of immobilized chimeric DNA ligations, library generation, sequencing, and genome-wide analysis of interactions. For complete details on the use and execution of this protocol, please refer to Andreu et al. (2022).1.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis work was supported by grants PID2020-115755GB-I00, BFU2017-84914-P, and BFU2015-72319-EXP to M.M.; PID2019-106499RB-I00 to A.L.; FJCI-2014-19847 to M.J.A.; and PRE2018-083477 to M.P., funded by MCIN/AEI/10.13039/501100011033. The CBMSO is supported by an institutional grant from the Fundacio´ n Ramo´ n Areces and is a Severo Ochoa Center of Excellence (grant CEX2021- 001154-S funded by MICIN/AEI/10.13039/501100011033). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovacio´ n (MCIN), and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S funded by MICIN/ AEI/10.13039/501100011033). The CNIO is a Severo Ochoa Center of Excellence (grant CEX2019-000891-S funded by MICIN/AEI/10.13039/501100011033).es_ES
dc.format.number2es_ES
dc.format.page102267es_ES
dc.format.volume4es_ES
dc.identifier.citationSTAR Protoc. 2023 Apr 30;4(2):102267.es_ES
dc.identifier.doi10.1016/j.xpro.2023.102267es_ES
dc.identifier.e-issn2666-1667es_ES
dc.identifier.journalSTAR protocolses_ES
dc.identifier.pubmedID37126445es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/19312
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2020-115755GB-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BFU2017-84914-Pes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BFU2015-72319-EXPes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-106499RB-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/FJCI-2014-19847es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PRE2018-083477es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/MICIN/AEI/10.13039/501100011033/CEX2020-001041-Ses_ES
dc.relation.publisherversion10.1016/j.xpro.2023.102267es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genómica Funcionales_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication3f396508-b13f-42cc-9e92-a3e7ff336db1
relation.isAuthorOfPublication56c10503-dda9-492f-86f2-9107563c6aab
relation.isAuthorOfPublicatione017210f-b6ce-4b88-8c4d-57d3ea201b7a
relation.isAuthorOfPublication.latestForDiscovery3f396508-b13f-42cc-9e92-a3e7ff336db1

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