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Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

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URI: http://hdl.handle.net/20.500.12105/11317
ISSN: 1550-7408
DOI: 10.1111/jeu.12772
Publication date
2020-03-01
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Wiley
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Abstract
The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.
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Subtypes Zoonotic transmission Sanger Next Generation Sequencing Wildlife
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J Eukaryot Microbiol. 2020 Mar;67(2):273-278.
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Centro Nacional de Microbiología (CNM)
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https://doi.org/10.1111/jeu.12772
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research article