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Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

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dc.contributor.authorCalero-Bernal, Rafael
dc.contributor.authorSantín, Mónica
dc.contributor.authorMaloney, Jenny G
dc.contributor.authorMartín-Pérez, Manuel
dc.contributor.authorHabela, Miguel Ángel
dc.contributor.authorFernández-García, José L
dc.contributor.authorFigueiredo, Ana
dc.contributor.authorNájera, Fernando
dc.contributor.authorPalacios, María J
dc.contributor.authorMateo, Marta
dc.contributor.authorBalseiro, Ana
dc.contributor.authorBarral, Marta
dc.contributor.authorLima-Barberoi, José F
dc.contributor.authorKöster, Pamela Carolina
dc.contributor.authorCarmena, David
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderComplutense University of Madrid (España)
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.date.accessioned2020-11-11T13:47:26Z
dc.date.available2020-11-11T13:47:26Z
dc.date.issued2020-03-01
dc.description.abstractThe occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis study was funded by the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain), under project PI16CIII/00024. Additional funding was obtained from the Complutense University of Madrid (grant CT65/16), the University of Aveiro (Portugal) and FCT/MEC through CESAM RU funding (UID/AMB/50017/2019), and European FEDER (PT2020 Agreement). The authors thank Aleksey Molokin for technical services in support of this study.es_ES
dc.format.number2es_ES
dc.format.page273-278es_ES
dc.format.volume67es_ES
dc.identifier.citationJ Eukaryot Microbiol. 2020 Mar;67(2):273-278.es_ES
dc.identifier.doi10.1111/jeu.12772es_ES
dc.identifier.issn1550-7408
dc.identifier.journalThe Journal of eukaryotic microbiologyes_ES
dc.identifier.pubmedID31691450es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11317
dc.language.isoenges_ES
dc.publisherWiley
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI16CIII/00024es_ES
dc.relation.publisherversionhttps://doi.org/10.1111/jeu.12772es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectSubtypeses_ES
dc.subjectZoonotic transmissiones_ES
dc.subjectSangeres_ES
dc.subjectNext Generation Sequencinges_ES
dc.subjectWildlifees_ES
dc.titleBlastocystis sp. Subtype Diversity in Wild Carnivore Species from Spaines_ES
dc.typeresearch articlees_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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