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Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.

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dc.contributor.authorDíaz-Díaz, Covadonga
dc.contributor.authorFernandez de Manuel, Laura
dc.contributor.authorJimenez-Carretero, Daniel
dc.contributor.authorMontoya, María Concepción
dc.contributor.authorClavería, Cristina
dc.contributor.authorTorres, Miguel
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrid (España)es_ES
dc.contributor.funderMinisterio de Educación, Cultura y Deporte (España)es_ES
dc.contributor.funderFundación ProCNICes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)es_ES
dc.date.accessioned2022-12-01T15:42:25Z
dc.date.available2022-12-01T15:42:25Z
dc.date.issued2017-09-25
dc.description.abstractThe mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed pluripotent state. During these events, epiblast cells and ESCs are prone to death, driven by competition between Myc-high cells (winners) and Myc-low cells (losers). Using live tracking of Myc levels, we show that Myc-high ESCs approach the naive pluripotency state, whereas Myc-low ESCs are closer to the differentiation-primed state. In ESC colonies, naive cells eliminate differentiating cells by cell competition, which is determined by a limitation in the time losers are able to survive persistent contact with winners. In the mouse embryo, cell competition promotes pluripotency maintenance by elimination of primed lineages before gastrulation. The mechanism described here is relevant to mammalian embryo development and induced pluripotency.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe thank B. Sleckman for the GFP-Myc mice, the CNIC Microscopy unit for the live confocal analysis, the CNIC Genomics and Bioinformatics units for the RNA-seq analysis, J.C. Alonso for video editing, and M. Manzanares, J.A. Enrı´quez, and members of the Torres lab for helpful comments on the manuscript. This work was supported by grants BFU2015-71519-P and RD16/0011/0019 (ISCIII) from the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) and grant P2010/BMD-2315 from the Madrid Regional Government to M.T., and grants (BIO2014-62200-EXP) and (PIFIS-2016) from MEIC to M.C.M. C.D.D. was supported by an FPU grant from the Spanish Ministry of Education, Culture and Sports. The CNIC is supported by the Spanish MEIC and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).es_ES
dc.format.number6es_ES
dc.format.page585es_ES
dc.format.volume42es_ES
dc.identifier.citationDev Cell 2017 Sep 25;42(6):585-599.e4es_ES
dc.identifier.doi10.1016/j.devcel.2017.08.011es_ES
dc.identifier.e-issn1878-1551es_ES
dc.identifier.journalDevelopmental celles_ES
dc.identifier.pubmedID28919206es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/15254
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BFU2015-71519-Pes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/RD16/0011/0019es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/P2010/BMD-2315es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BIO2014-62200-EXPes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/PIFIS-2016es_ES
dc.relation.publisherversion10.1016/j.devcel.2017.08.011es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Control Genético del Desarrollo y Regeneración de Órganoses_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshCell Differentiationes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Communicationes_ES
dc.subject.meshCell Lineagees_ES
dc.subject.meshCell Proliferationes_ES
dc.subject.meshCell Survivales_ES
dc.subject.meshCell Trackinges_ES
dc.subject.meshCells, Culturedes_ES
dc.subject.meshEmbryo, Mammalianes_ES
dc.subject.meshGastrulationes_ES
dc.subject.meshGene Expression Profilinges_ES
dc.subject.meshGerm Layerses_ES
dc.subject.meshInheritance Patternses_ES
dc.subject.meshMicees_ES
dc.subject.meshMouse Embryonic Stem Cellses_ES
dc.subject.meshPluripotent Stem Cellses_ES
dc.subject.meshProto-Oncogene Proteins c-myces_ES
dc.subject.meshTime Factorses_ES
dc.titlePluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication35c9d189-273f-49b9-b5f6-43788016b2ca
relation.isAuthorOfPublication6ec1130e-9194-41d3-b53f-eba5fc1af5c9
relation.isAuthorOfPublication.latestForDiscovery35c9d189-273f-49b9-b5f6-43788016b2ca
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