Publication: Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
Loading...
Identifiers
Publication date
2017-09-25
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Metrics
Export
Abstract
The mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed pluripotent state. During these events, epiblast cells and ESCs are prone to death, driven by competition between Myc-high cells (winners) and Myc-low cells (losers). Using live tracking of Myc levels, we show that Myc-high ESCs approach the naive pluripotency state, whereas Myc-low ESCs are closer to the differentiation-primed state. In ESC colonies, naive cells eliminate differentiating cells by cell competition, which is determined by a limitation in the time losers are able to survive persistent contact with winners. In the mouse embryo, cell competition promotes pluripotency maintenance by elimination of primed lineages before gastrulation. The mechanism described here is relevant to mammalian embryo development and induced pluripotency.
Description
Keywords
MeSH Terms
Cell Differentiation Animals Cell Communication Cell Lineage Cell Proliferation Cell Survival Cell Tracking Cells, Cultured Embryo, Mammalian Gastrulation Gene Expression Profiling Germ Layers Inheritance Patterns Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Proto-Oncogene Proteins c-myc Time Factors
DeCS Terms
Bibliographic citation
Dev Cell 2017 Sep 25;42(6):585-599.e4