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Identification of small molecules capable of enhancing viral membrane fusion

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dc.contributor.authorGarcía-Murria, Mª Jesús
dc.contributor.authorGadea-Salom, Laura
dc.contributor.authorMoreno, Sandra
dc.contributor.authorRius-Salvador, Marina
dc.contributor.authorZaragoza, Oscar
dc.contributor.authorBrun, Alejandro
dc.contributor.authorMingarro, Ismael
dc.contributor.authorMartínez-Gil, Luis
dc.contributor.funderGeneralitat Valenciana (España)es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderUnión Europea. Comisión Europea. H2020es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.date.accessioned2023-06-06T11:07:25Z
dc.date.available2023-06-06T11:07:25Z
dc.date.issued2023-05-24
dc.description.abstractSeveral approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis work was supported by the Generalitat Valenciana (PROMETEO/2019/065) and grant PID2020-119111GB-I00 by MCIN/AEI/10.13039/501100011033. O.Z. is funded by grant PID2020-114546RB by MCIN/AEI/10.13039/501100011033. L.G. is the recipient of a predoctoral contract (CIACIF/2021/119) from the Generatitat Valenciana. M.R-S is the recipient of a predoctoral contract from the Spanish Ministry of Science and Innovation (PRE2021-101042 by MCIN/AEI/10.13039/501100011033). This publication was also supported by the European Virus Archive GLOBAL (EVA-GLOBAL) project which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement 871029.es_ES
dc.format.number1es_ES
dc.format.page99es_ES
dc.format.volume20es_ES
dc.identifier.citationVirol J. 2023 May 24;20(1):99.es_ES
dc.identifier.doi10.1186/s12985-023-02068-1es_ES
dc.identifier.e-issn1743-422Xes_ES
dc.identifier.journalVirology journales_ES
dc.identifier.pubmedID37226231es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16143
dc.language.isoenges_ES
dc.publisherBioMed Central (BMC)es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2020-119111GB-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2020-114546RBes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PRE2021-101042es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/871029/EUes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEnveloped viruses_ES
dc.subjectHTSes_ES
dc.subjectHigh-throughput screeninges_ES
dc.subjectInfluenza a viruses_ES
dc.subjectMembrane fusiones_ES
dc.subjectNipah viruses_ES
dc.subjectSARS-CoV-2es_ES
dc.subjectSyncytiumes_ES
dc.subjectViral entryes_ES
dc.subject.meshCOVID-19es_ES
dc.subject.meshHumanses_ES
dc.subject.meshSARS-CoV-2es_ES
dc.subject.meshVirus Internalizationes_ES
dc.subject.meshBiological Assayes_ES
dc.subject.meshGene Libraryes_ES
dc.titleIdentification of small molecules capable of enhancing viral membrane fusiones_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication298933e5-bfff-4e88-83ec-e4d2cb6581e1
relation.isAuthorOfPublication.latestForDiscovery298933e5-bfff-4e88-83ec-e4d2cb6581e1

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