Publication:
Identification of small molecules capable of enhancing viral membrane fusion

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URI: http://hdl.handle.net/20.500.12105/16143
DOI: 10.1186/s12985-023-02068-1
Publication date
2023-05-24
Authors
García-Murria, Mª Jesús
Gadea-Salom, Laura
Moreno, Sandra
Rius-Salvador, Marina
Brun, Alejandro
Mingarro, Ismael
Martínez-Gil, Luis
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BioMed Central (BMC)
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Abstract
Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.
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Keywords
Enveloped virus HTS High-throughput screening Influenza a virus Membrane fusion Nipah virus SARS-CoV-2 Syncytium Viral entry
MeSH Terms
COVID-19 Humans SARS-CoV-2 Virus Internalization Biological Assay Gene Library
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Virol J. 2023 May 24;20(1):99.
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Centro Nacional de Microbiología (CNM)
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journal article