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Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration

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dc.contributor.authorSanchez-Iranzo, Hector
dc.contributor.authorGalardi-Castilla, Maria
dc.contributor.authorMinguillon, Carolina
dc.contributor.authorSanz-Morejon, Andres
dc.contributor.authorGonzalez-Rosa, Juan Manuel
dc.contributor.authorFelker, Anastasia
dc.contributor.authorErnst, Alexander
dc.contributor.authorGuzman-Martinez, Gabriela
dc.contributor.authorMosimann, Christian
dc.contributor.authorMercader, Nadia
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderSwiss National Science Foundation
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)
dc.contributor.funderUnión Europea. Comisión Europea
dc.contributor.funderFundación ProCNIC
dc.contributor.funderSwiss Heart Foundation
dc.date.accessioned2018-11-22T08:10:54Z
dc.date.available2018-11-22T08:10:54Z
dc.date.issued2018
dc.description.abstractDuring development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a(+) cells, with a small contribution from tbx5a(+) SHF progenitors. Notably, ablation of ventricular tbx5a(+)-derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells. In the adult, tbx5a expression is restricted to the trabeculae and excluded from the outer cortical layer. tbx5a-lineage tracing revealed that trabecular cardiomyocytes can switch their fate and differentiate into cortical myocardium during adult heart regeneration. We conclude that a high degree of cardiomyocyte cell fate plasticity contributes to efficient regeneration.
dc.description.peerreviewedSí
dc.description.sponsorshipWe are grateful to the Animal facility, Histology, Microscopy, Cellomics, Bioinformatics, and Genomics Units from CNIC, the Microscopy Imaging Center from the University of Bern; X. Langa, L. Flores, M. Villalba, and R. Costa for experimental assistance; S. Seyfried and C.-B. Chien for reagents; and M. Torres, S. Martin-Puig, J. Gonzalez-Sainz-de-Aja, R. M. Benedito, and A. Jazwinska for discussion. Funding was through FPU12/03007 and BFU2014-56970-P (Plan Estatal de Investigacion Cientifica y Tecnica y de Innovacion 2013-2016, Programa Estatal de I+D+i Orientada a los Retos de la Sociedad Retos Investigacion: Proyectos I+D+i 2016, del Ministerio de Economia competitividad e Industria), and co-funding by Fondo Europeo de Desarrollo Regional (FEDER) (H.S. and N.M.); Swiss National Science Foundation grant 31003A\_159721, ANR-SNF collaborative Project 320030E-164245, and the ERC starting grant 337703-zebra-Heart (N.M.). A.E. is enrolled into PhD specialization Cutting Edge Microscopy offered by the Graduate School for Cellular and Biomedical Sciences (GCB) and the Microscopy Imaging Center (MIC). The CNIC is supported by the Ministry of Economy, Industry and Competitiveness (MEIC) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505). Further support was from the Canton of Zurich, project grant from the Swiss Heart Foundation (A.F. and C. Mosimann); the Swiss National Science Foundation (SNSF) professorship (PP00P3\_139093) and a Marie Curie Career Integration Grant from the European Commission (CIG PCIG14-GA-2013- 631984) (C. Mosimann).
dc.format.volume9
dc.identifierISI:000423510600005
dc.identifier.citationNat Commun. 2018; 9(1):428
dc.identifier.doi10.1038/s41467-017-02650-6
dc.identifier.issn2041-1723
dc.identifier.journalNature Communications
dc.identifier.pubmedID29382818
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6693
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/337703/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/631984/EUes_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-017-02650-6
dc.repisalud.institucionCNIC
dc.repisalud.orgCNICCNIC::Grupos de investigación::Desarrollo del Epicardio y su Papel en la Regeneración
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleTbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration
dc.typejournal article
dc.type.hasVersionVoR
dspace.entity.typePublication
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