Publication:
A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

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dc.contributor.authorHanke, Leo
dc.contributor.authorDas, Hrishikesh
dc.contributor.authorSheward, Daniel J.
dc.contributor.authorPerez Vidakovics, Laura
dc.contributor.authorUrgard, Egon
dc.contributor.authorMoliner-Morro, Ainhoa
dc.contributor.authorKim, Changil
dc.contributor.authorKarl, Vivien
dc.contributor.authorPankow, Alec
dc.contributor.authorSmith, Natalie L.
dc.contributor.authorPorebski, Bartlomiej
dc.contributor.authorFernandez-Capetillo, Oscar
dc.contributor.authorSezgin, Erdinc
dc.contributor.authorPedersen, Gabriel K.
dc.contributor.authorCoquet, Jonathan M.
dc.contributor.authorHällberg, B. Martin
dc.contributor.authorMurrell, Ben
dc.contributor.authorMcInerney, Gerald M.
dc.contributor.funderVetenskapsradet (Swedish Research Council)es_ES
dc.contributor.funderKnut and Alice Wallenberg Foundation
dc.contributor.funderKarolinska Institutet
dc.contributor.funderUnión Europea. Comisión Europea. H2020
dc.date.accessioned2023-05-17T09:15:01Z
dc.date.available2023-05-17T09:15:01Z
dc.date.issued2022-01-13
dc.description.peerreviewedNoes_ES
dc.description.sponsorshipExperiments with replication-competent SARS-CoV-2 were performed in the Biomedicum BSL3 core facility, Karolinska Institutet. We thank Jonas Klingström for providing Calu-3 cells and sharing the Swedish SARS-CoV-2 isolate, and Alex Sigal from the Africa Health Research Institute for providing the beta variant (B.1.351/501Y.V2) isolate. We thank Penny Moore and the NICD (South Africa) for providing the B.1.351/beta variant spike plasmid, which was generated using funding from the South African Medical Research Council. We gratefully acknowledge the G2P-UK National Virology consortium funded by MRC/UKRI (grant ref: MR/W005611/1.) and the Barclay Lab at Imperial College for providing the B.1.617.2 spike plasmid. All cryo-EM data were collected in the Karolinska Institutet’s 3D-EM facility. We thank Agustin Ure for assistance with figure generation and Tomas Nyman (Protein Science Facility at KI) for providing access to SPR instruments. L.H. was supported by the David och Astrid Hageléns stiftelse, the Clas Groschinskys Minnesfond and a Jonas Söderquist’s scholarship. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 101003653 (CoroNAb), to B.M. and G.M.M. B.M.H. is supported by the Knut and Alice Wallenberg Foundation (KAW 2017.0080 and KAW 2018.0080). The work was supported by project grants from the Swedish Research Council to E.S. (2020-02682), B.M.H. (2017-6702 and 2018-3808), B.M. (2018-02381) and to G.M.M. (2018-03914 and 2018-03843). E.S. is supported by Karolinska Institutet Foundation Grants, National Molecular Medicine Program Grants, and the grants from the SciLifeLab National COVID-19 Research Program, financed by the Knut and Alice Wallenberg Foundation. We thank National Microscopy Infrastructure, NMI (VR-RFI 2016-00968).es_ES
dc.format.number1es_ES
dc.format.volume13es_ES
dc.identifier.citationNat Commun. 2022;13(1):155.es_ES
dc.identifier.doi10.1038/s41467-021-27610-zes_ES
dc.identifier.e-issn2041-1723es_ES
dc.identifier.journalNature Communicationses_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16073
dc.language.isoenges_ES
dc.publisherNature Publishing Group
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-021-27610-z.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Inestabilidad Genómicaes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalses_ES
dc.subject.meshAntibodies, Bispecifices_ES
dc.subject.meshAntibodies, Neutralizinges_ES
dc.subject.meshAntibodies, Virales_ES
dc.subject.meshCOVID-19es_ES
dc.subject.meshChlorocebus aethiopses_ES
dc.subject.meshCryoelectron Microscopyes_ES
dc.subject.meshHEK293 Cellses_ES
dc.subject.meshHumanses_ES
dc.subject.meshMice, Transgenices_ES
dc.subject.meshNeutralization Testses_ES
dc.subject.meshProtein Bindinges_ES
dc.subject.meshProtein Conformationes_ES
dc.subject.meshProtein Multimerizationes_ES
dc.subject.meshSARS-CoV-2es_ES
dc.subject.meshSingle-Domain Antibodieses_ES
dc.subject.meshSpike Glycoprotein, Coronaviruses_ES
dc.subject.meshVero Cellses_ES
dc.titleA bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivoes_ES
dc.typejournal articlees_ES
dc.type.hasVersionSMURes_ES
dspace.entity.typePublication
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