Publication:
A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.

Simple item page
dc.contributor.authorNeupane, Ritam
dc.contributor.authorPisareva, Vera P
dc.contributor.authorRodriguez, Carlos F
dc.contributor.authorPisarev, Andrey V
dc.contributor.authorFernández, Israel S
dc.contributor.funderUnited States Department of Health and Human Services
dc.date.accessioned2020-09-04T12:35:43Z
dc.date.available2020-09-04T12:35:43Z
dc.date.issued2020-04-14
dc.description.abstractTaking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have evolved. The initiation stage of translation is especially targeted as it involves multiple steps and the engagement of numerous initiation factors. The use of structured RNA sequences, called Internal Ribosomal Entry Sites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5'-UTR of a viral RNA assembles a functional initiation complex via an uAUG intermediate. The IRES features a novel extended, multi-domain architecture, that circles the 40S head. The structures and accompanying functional data illustrate the importance of 5'-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipColumbia University Start package Israel S Fernandez National Institute of General Medical Sciences GM097014 Andrey V Pisarev The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.es_ES
dc.format.volume9es_ES
dc.identifier.citationElife . 2020 ;9:e54575.es_ES
dc.identifier.doi10.7554/eLife.54575es_ES
dc.identifier.e-issn2050-084Xes_ES
dc.identifier.journaleLifees_ES
dc.identifier.pubmedID32286223es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10981
dc.language.isoenges_ES
dc.publishereLife Sciences Publicationses_ES
dc.relation.publisherversionhttps://doi.org/10.7554/eLife.54575.es_ES
dc.repisalud.institucionCNIOes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectEUKARYOTIC TRANSLATION INITIATIONes_ES
dc.subjectOPEN READING FRAMESes_ES
dc.subjectCRYO-EM STRUCTUREes_ES
dc.subjectSCANNING MECHANISMes_ES
dc.subjectRIBOSOMEes_ES
dc.subjectVIRUSes_ES
dc.subjectPROTEINes_ES
dc.subjectREINITIATIONes_ES
dc.subjectELONGATIONes_ES
dc.subjectREFINEMENTes_ES
dc.titleA complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AcomplexIRESatthe5’-UTR_2020.pdf
Size:
6.35 MB
Format:
Adobe Portable Document Format
Description:
Artículo principal
Download
Collections
Grupos de investigación