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MitoBlue as a tool to analyze the mitochondria-lysosome communication

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dc.contributor.authorSánchez, Mateo I.
dc.contributor.authorVida, Yolanda
dc.contributor.authorPérez-Inestrosa, Ezequiel
dc.contributor.authorMascareñas, José L.
dc.contributor.authorVázquez, M. Eugenio
dc.contributor.authorSugiura, Ayumu
dc.contributor.authorMartínez-Costas, José
dc.contributor.authoraffiliation[Sánchez,MI; Mascareñas,JL; Vázquez,ME] Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, Santiago de Compostela, Spain. [Sugiura,A] Montreal Neurological Institute, McGill University, Montreal, QC, Canada. [Sugiura,A] Department of Neurology and Neurosurgery, McGill University, Montreal, QC, Canada. [Vida,Y; Pérez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología-BIONAND. Campanillas, Málaga, Spain. [Martínez-Costas,J] Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Bioquímica y Biología Molecular, Universidade de Santiago de Compostela, Santiago de, Compostela, Spain. [Vida,Y; Pérez-Inestrosa,E] Universidad de Málaga-IBIMA, Departamento de Química Orgánica, Málaga, Spain.
dc.date.accessioned2024-02-12T19:45:28Z
dc.date.available2024-02-12T19:45:28Z
dc.date.issued2020-02-26
dc.description.abstractMitoBlue is a fluorescent bisamidine that can be used to easily monitor the changes in mitochondrial degradation processes in different cells and cellular conditions. MitoBlue staining pattern is exceptional among mitochondrial dyes and recombinant fluorescent probes, allowing the dynamic study of mitochondrial recycling in a variety of situations in living cells. MitoBlue is a unique tool for the study of these processes that will allow the detailed characterization of communication between mitochondria and lysosomes.
dc.description.sponsorshipThe support was given by the Spanish grants BFU2013-43513-R, SAF2016-76689-R, CTQ2015-70698-R, RTI2018-099877-B-I00, CTQ2013-49317-EXP, CTQ2013-41339-P, CTQ2016-75870-P, CTQ2015-71896-REDT (Red de Fotoquímica Biológica) and Instituto de Salud Carlos III (ISCIII; RETIC ARADYAL D16/0006/0012), Junta de Andalucía (PI0250-2016 and UMA18-FEDERJA-007), Orfeo-cinqa network CTQ2016-81797-REDC; the Xunta de Galicia (2015-CP082, ED431C 2017/19, Centro Singular de investigación de Galicia accreditation 2019–2022, ED431G 2019/03), the European Union (European Regional Development Fund - ERDF), and the European Research Council (Advanced Grant No. 340055) are gratefully acknowledged. M.E.V. also acknowledges the support provided by the Fundación AECC IDEAS197VAZQ grant). A.S. was supported by the Uehara Memorial Foundation and a JSPS Postdoctoral Fellowship for Research Abroad.
dc.identifier.doi10.1038/s41598-020-60573-7
dc.identifier.e-issn2045-2322es_ES
dc.identifier.journalScientific Reportses_ES
dc.identifier.otherhttp://hdl.handle.net/10668/3856
dc.identifier.pubmedID32103132es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/18006
dc.language.isoeng
dc.publisherSpringer
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-020-60573-7es
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMitochondrial degradation
dc.subjectLysosome
dc.subjectMicroscopy
dc.subjectMitochondria
dc.subjectProteins
dc.subjectQuality control
dc.subjectMitofagia
dc.subjectLisosomas
dc.subjectMicroscopía
dc.subjectMitocondrias
dc.subjectProteínas
dc.subjectControl de calidad
dc.subject.mesh2-Naphthylamine
dc.subject.meshAmidines
dc.subject.meshAnimals
dc.subject.meshChick Embryo
dc.subject.meshFibroblasts
dc.subject.meshLysosomes
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshMitochondria
dc.subject.meshFluorescent Dyes
dc.titleMitoBlue as a tool to analyze the mitochondria-lysosome communication
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication
relation.isPublisherOfPublication8d558850-2ef2-4d1e-b0e1-4e5591ab6288
relation.isPublisherOfPublication.latestForDiscovery8d558850-2ef2-4d1e-b0e1-4e5591ab6288

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