Publication: Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins
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2022-03-14
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Hsieh, Ching-Lin
Rush, Scott A
Palomo-Sanz, Concepcion ISCIII 
Chou, Chia-Wei
Pickens, Whitney
Mas-Lloret, Vicente ISCIII 
McLellan, Jason S
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Nature Publishing Group
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Abstract
The human metapneumovirus (hMPV) fusion (F) protein is essential for viral entry and is a key target of neutralizing antibodies and vaccine development. The prefusion conformation is thought to be the optimal vaccine antigen, but previously described prefusion F proteins expressed poorly and were not well stabilized. Here, we use structures of hMPV F to guide the design of 42 variants containing stabilizing substitutions. Through combinatorial addition of disulfide bonds, cavity-filling substitutions, and improved electrostatic interactions, we describe a prefusion-stabilized F protein (DS-CavEs2) that expresses at 15 mg/L and has a melting temperature of 71.9 °C. Crystal structures of two prefusion-stabilized hMPV F variants reveal that antigenic surfaces are largely unperturbed. Importantly, immunization of mice with DS-CavEs2 elicits significantly higher neutralizing antibody titers against hMPV A1 and B1 viruses than postfusion F. The improved properties of DS-CavEs2 will advance the development of hMPV vaccines and the isolation of therapeutic antibodies.
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Nat Commun. 2022 Mar 14;13(1):1299.