Browsing by Keyword "Nipah virus"
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Publication Identification of small molecules capable of enhancing viral membrane fusion(BioMed Central (BMC), 2023-05-24) García-Murria, Mª Jesús; Gadea-Salom, Laura; Moreno, Sandra; Rius-Salvador, Marina; Zaragoza, Oscar; Brun, Alejandro; Mingarro, Ismael; Martínez-Gil, Luis; Generalitat Valenciana (España); Ministerio de Ciencia e Innovación (España); Unión Europea. Comisión Europea. H2020; Agencia Estatal de Investigación (España)Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.