Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7890
Epigenomic Co-localization and Co-evolution Reveal a Key Role for 5hmC as a Communication Hub in the Chromatin Network of ESCs
Cell Rep. 2016;14(5):1246-1257
Epigenetic communication through histone and cytosine modifications is essential for gene regulation and cell identity. Here, we propose a framework that is based on a chromatin communication model to get insight on the function of epigenetic modifications in ESCs. The epigenetic communication network was inferred from genome-wide location data plus extensive manual annotation. Notably, we found that 5-hydroxymethylcytosine (5hmC) is the most-influential hub of this network, connecting DNA demethylation to nucleosome remodeling complexes and to key transcription factors of pluripotency. Moreover, an evolutionary analysis revealed a central role of 5hmC in the co-evolution of chromatin-related proteins. Further analysis of regions where 5hmC co-localizes with specific interactors shows that each interaction points to chromatin remodeling, stemness, differentiation, or metabolism. Our results highlight the importance of cytosine modifications in the epigenetic communication of ESCs.
5-Methylcytosine | Animals | Chromatin | Cytosine | Mice | Mouse Embryonic Stem Cells | Multiprotein Complexes | Signal Transduction | Epigenesis, Genetic | Evolution, Molecular
Files in this item
- EpigenomicCo-localizationandCo ...