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dc.contributor.authorPutri, Rindia M.
dc.contributor.authorAllende-Ballestero, Carolina
dc.contributor.authorLuque, Daniel 
dc.contributor.authorKlem, Robin
dc.contributor.authorRousou, Katerina-Asteria
dc.contributor.authorLiu, Aijie
dc.contributor.authorTraulsen, Christoph H.-H.
dc.contributor.authorRurup, W. Frederik
dc.contributor.authorKoay, Melissa S. T.
dc.contributor.authorCastón, José R.
dc.contributor.authorCornelissen, Jeroen J. L. M.
dc.date.accessioned2019-02-27T18:26:16Z
dc.date.available2019-02-27T18:26:16Z
dc.date.issued2017-11-22
dc.identifier.citationACS Nano. 2017;26;11(12):12796-12804.es_ES
dc.identifier.issn1936-0851es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7245
dc.description.abstractRecent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo, resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM). Furthermore, by harnessing the confined enzyme (i.e., a peroxidase), we demonstrate the functionality of the encapsulin for an in vitro surface-immobilized catalysis in a cascade pathway with an additional enzyme, glucose oxidase. We also demonstrate the in vivo functionality of the encapsulin for cellular uptake using mammalian macrophages. Unraveling both the structure and functionality of the encapsulins allows transforming biological nanocompartments into functional systems.es_ES
dc.description.sponsorshipWe acknowledge financial support from the ERC Consolidator Grant (Protcage) and the Indonesia Endowment Fund for Education (LPDP). This work was supported in part by grants from the Spanish Ministry of Economy and Competitivity (BFU2014-55475 to J.R.C.) and the Comunidad Autónoma de Madrid (S2013/MIT-2807 to J.R.C.).es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBacterial compartmentses_ES
dc.subjectCryo-electron microscopyes_ES
dc.subjectEncapsulines_ES
dc.subjectNanoplatformses_ES
dc.subjectNanoreactorses_ES
dc.titleStructural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptakees_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución- 4.0 Internacional*
dc.identifier.pubmedID29166561es_ES
dc.format.volume11es_ES
dc.format.number12es_ES
dc.format.page12804es_ES
dc.identifier.doi10.1021/acsnano.7b07669es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderIndonesia Endowment Fund forEducation (LPDP)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad Autónoma de Madrides_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1936-086Xes_ES
dc.relation.publisherversionhttps://www.doi.org/10.1021/acsnano.7b07669es_ES
dc.identifier.journalACS Nanoes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución- 4.0 Internacional
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