Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/19907
Título
Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment.
Autor(es)
Ugalde-Triviño, Lola | Molina-Jiménez, Francisca | H-Vázquez, Juan | Relaño-Rupérez, Carlos | Arias-González, Laura | Casabona, Sergio | Pérez-Fernández, María Teresa | Martín-Domínguez, Verónica | Fernández-Pacheco, Jennifer | Lucendo, Alfredo J | Bernardo, David | Santander, Cecilio | Majano, Pedro
Fecha de publicación
2024
Cita
Front Immunol. 2024 Apr 5:15:1374611.
Idioma
Inglés
Tipo de documento
journal article
Resumen
OBJECTIVES
The aim of the study was to characterize the circulating immunome of patients with EoE before and after proton pump inhibitor (PPI) treatment in order to identify potential non-invasive biomarkers of treatment response.
METHODS
PBMCs from 19 healthy controls and 24 EoE patients were studied using a 39-plex spectral cytometry panel. The plasmacytoid dendritic cell (pDC) population was differentially characterized by spectral cytometry analysis and immunofluorescence assays in esophageal biopsies from 7 healthy controls and 13 EoE patients.
RESULTS
Interestingly, EoE patients at baseline had lower levels of circulating pDC compared with controls. Before treatment, patients with EoE who responded to PPI therapy had higher levels of circulating pDC and classical monocytes, compared with non-responders. Moreover, following PPI therapy pDC levels were increased in all EoE patients, while normal levels were only restored in PPI-responding patients. Finally, circulating pDC levels inversely correlated with peak eosinophil count and pDC count in esophageal biopsies. The number of tissue pDCs significantly increased during active EoE, being even higher in non-responder patients when compared to responder patients pre-PPI. pDC levels decreased after PPI intake, being further restored almost to control levels in responder patients post-PPI.
CONCLUSIONS
We hereby describe a unique immune fingerprint of EoE patients at diagnosis. Moreover, circulating pDC may be also used as a novel non-invasive biomarker to predict subsequent response to PPI treatment.
MESH
Proton Pump Inhibitors | Eosinophilic Esophagitis | Biomarkers | Dendritic Cells | Humans | Male | Female | Adult | Middle Aged | Eosinophils | Treatment Outcome | Young Adult | Biopsy | Case-Control Studies
Versión en línea
DOI
Aparece en las colecciones
Ficheros en el ítem
- Nombre:
- Circulating immunome fingerpri ...
- Tamaño:
- 10.32Mb
- Formato:
- Descripción:
- Artículo