Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16261
Título
Positron Emission Tomography-Computed Tomography and Magnetic Resonance Imaging Assessments in a Mouse Model of Implant-Related Bone and Joint Staphylococcus aureus Infection.
Autor(es)
Aguilera-Correa, JJ | Salinas, B | González-Arjona, M | de Pablo, D | Muñoz, P | Bouza, E | Fernández Aceñero, M J | Esteban, J | Desco, Manuel CNIC | Cussó, L | Aguilera-Correa, J. J. | Salinas, B. | González-Arjona, M. | de Pablo, D. | Muñoz, P. | Bouza, E. | Fernández Aceñero, M. J. | Esteban, J. | Desco, M. | Cussó, L.
Fecha de publicación
2023-06-15
Cita
Microbiol Spectr. 2023 Jun 15;11(3):e0454022
Idioma
Inglés
Tipo de documento
journal article
Resumen
Osteomyelitis is an infection of the bone, associated with an inflammatory process. Imaging plays an important role in establishing the diagnosis and the most appropriate patient management. However, data are lacking regarding the use of preclinical molecular imaging techniques to assess osteomyelitis progression in experimental models. This study aimed to compare structural and molecular imaging to assess disease progression in a mouse model of implant-related bone and joint infections caused by Staphylococcus aureus. In SWISS mice, the right femur was implanted with a resorbable filament impregnated with S. aureus (infected group, n = 10) or sterile culture medium (uninfected group, n = 6). Eight animals (5 infected, 3 uninfected) were analyzed with magnetic resonance imaging (MRI) at 1, 2, and 3 weeks postintervention, and 8 mice were analyzed with [18F]fluorodeoxyglucose (FDG)-positron emission tomography (PET)-computed tomography (CT) at 48 h and at 1, 2, and 3 weeks postintervention. In infected animals, CT showed bone lesion progression, mainly in the distal epiphysis, although some uninfected animals presented evident bone sequestra at 3 weeks. MRI showed a lesion in the articular area that persisted for 3 weeks in infected animals. This lesion was smaller and less evident in the uninfected group. At 48 h postintervention, FDG-PET showed higher joint uptake in the infected group than in the uninfected group (P = 0.025). Over time, the difference between groups increased. These results indicate that FDG-PET imaging was much more sensitive than MRI and CT for differentiating between infection and inflammation at early stages. FDG-PET clearly distinguished between infection and postsurgical bone healing (in uninfected animals) from 48 h to 3 weeks after implantation. IMPORTANCE Our results encourage future investigations on the utility of the model for testing different therapeutic procedures for osteomyelitis.
MESH
Staphylococcal Infections | Osteomyelitis | Animals | Mice | Positron Emission Tomography Computed Tomography | Fluorodeoxyglucose F18 | Staphylococcus aureus | Magnetic Resonance Imaging
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