Publication:
Assaying chromatin sirtuins.

Simple item page
dc.contributor.authorZhong, Lei
dc.contributor.authorMartinez-Pastor, Barbara
dc.contributor.authorSilberman, Dafne M
dc.contributor.authorSebastian, Carlos
dc.contributor.authorMostoslavsky, Raul
dc.date.accessioned2025-01-31T11:48:56Z
dc.date.available2025-01-31T11:48:56Z
dc.date.issued2013
dc.description.abstractMost of the sirtuins' nuclear substrates identified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins' substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors.
dc.format.page149-163
dc.format.volume1077
dc.identifier.citationMethods Mol Biol . 2013:1077:149-163
dc.identifier.journalMethods in Mol Biol
dc.identifier.pmchttps://pmc.ncbi.nlm.nih.gov/articles/PMC4605221/pdf/nihms-725238.pdf
dc.identifier.pubmedID24014405
dc.identifier.urihttps://hdl.handle.net/20.500.12105/26216
dc.language.isoeng
dc.publisherSpringuer
dc.relation.publisherversionhttps:// doi: 10.1007/978-1-62703-637-5_10.
dc.repisalud.institucionCNIO
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Microambiente y Metástasis
dc.rights.accessRightsopen access
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSirtuins
dc.subjectChromatin
dc.subjectChromatin immunoprecipitation
dc.subjectChIP
dc.subjectAcid-extraction
dc.subjectNucleosomal immunoprecipitation
dc.titleAssaying chromatin sirtuins.
dc.typeresearch article
dc.type.hasVersionAM
dspace.entity.typePublication

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
assayingchromatin-2013.pdf
Size:
7.15 MB
Format:
Adobe Portable Document Format
Download

Collections

Grupos de investigación