Publication:
miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p

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URI: http://hdl.handle.net/20.500.12105/5111
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-017-03689-7
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2017
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Nature Publishing Group
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microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
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MESSENGER-RNA MICRORNA EXPRESSION DIFFERENTIATION PI3K PREDICTION P85-ALPHA DUPLEXES DICER
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Sci Rep. 2017; 7(1):3508
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https://doi.org/10.1038/s41598-017-03689-7
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journal article