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Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis

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dc.contributor.authorPontes-Quero, Samuel
dc.contributor.authorHeredia, Luis
dc.contributor.authorCasquero-Garcia, Veronica
dc.contributor.authorFernandez-Chacon, Macarena
dc.contributor.authorLuo, Wen
dc.contributor.authorHermoso, Ana
dc.contributor.authorBansal, Mayank
dc.contributor.authorGarcia-Gonzalez, Irene
dc.contributor.authorSanchez-Munoz, Maria S.
dc.contributor.authorPerea, Juan R.
dc.contributor.authorGaliana-Simal, Adrian
dc.contributor.authorRodriguez-Arabaolaza, Iker
dc.contributor.authorDel Olmo-Cabrera, Sergio
dc.contributor.authorRocha, Susana
dc.contributor.authorCriado-Rodriguez, Luis M.
dc.contributor.authorGiovinazzo, Giovanna
dc.contributor.authorBenedito, Rui
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)
dc.contributor.funderFundación La Caixa
dc.contributor.funderFundación ProCNIC
dc.date.accessioned2017-10-20T10:23:09Z
dc.date.available2017-10-20T10:23:09Z
dc.date.issued2017
dc.description.abstractImproved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.
dc.description.peerreviewedSí
dc.description.sponsorshipThis work was supported by grants to the PI R.B. from the Spanish Ministry of Economy, Industry and Competitiveness (SAF2013-44329-P, SAF2013-42359-ERC, and RYC-2013-13209) and European Research Council (ERC-2014-StG - 638028). S.P.-Q., M.F.-C., and I.G.-G. were supported by PhD fellowships from Fundacion La Caixa (CX-SO-2013-02, CX\_E-2015-01, and CX-SO-16-1, respectively). W.L. by a FP7-PEOPLE-2012-COFUND GA600396 postdoctoral contract. We thank Simon Bartlett for English editing, Ralf H. Adams for sharing the Cdh5(PAC)-CreERT2 mice, Jose Luis de La Pompa for comments throughout the project and for sharing the Tie2-Cre mice, Gonzalo Gancedo for the help with the mouse colony, Valeria Caiolfa for the help with the microscopy, and all the members of the CNIC gene targeting, transgenesis, cellomics, and microscopy units. The CNIC is supported by MEIC/MINECO and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505).
dc.format.page800+
dc.format.volume170
dc.identifierISI:000407445700019
dc.identifier.citationCell. 2017; 170(4):800-814 e18
dc.identifier.doi10.1016/j.cell.2017.07.031
dc.identifier.e-issn1097-4172
dc.identifier.issn0092-8674
dc.identifier.journalCell
dc.identifier.pubmedID28802047
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5100
dc.language.isoeng
dc.publisherCell Press
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-44329-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-42359-ERCes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2013-13209es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/600396/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/638028/EUes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.cell.2017.07.031
dc.repisalud.institucionCNIC
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genética Funcional del Sistema de Fosforilación Oxidativa
dc.repisalud.orgCNICCNIC::Unidades técnicas::Transgénesis
dc.repisalud.orgCNICCNIC::Unidades técnicas::Células Pluripotentes
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCRE-LOXP
dc.subjectIN-VIVO
dc.subjectMOUSE
dc.subjectMICE
dc.subjectEXPRESSION
dc.subjectRECOMBINATION
dc.subjectSYSTEM
dc.titleDual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
dc.typejournal article
dc.type.hasVersionVoR
dspace.entity.typePublication
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