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PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.

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dc.contributor.authorSá da Bandeira, Diana
dc.contributor.authorKilpatrick, Alastair Morris
dc.contributor.authorMarques, Madalena
dc.contributor.authorGomez-Salazar, Mario
dc.contributor.authorVentura, Telma
dc.contributor.authorGonzalez, Zaniah Nashira
dc.contributor.authorStefancova, Dorota
dc.contributor.authorRossi, Fiona
dc.contributor.authorVermeren, Matthieu
dc.contributor.authorVink, Chris Sebastiaan
dc.contributor.authorBeltran, Mariana
dc.contributor.authorHenderson, Neil Cowan
dc.contributor.authorJung, Bongnam
dc.contributor.authorvan der Linden, Reinier
dc.contributor.authorvan de Werken, Harmen Jan George
dc.contributor.authorvan Ijcken, Wilfred F J
dc.contributor.authorBetsholtz, Christer
dc.contributor.authorForbes, Stuart John
dc.contributor.authorCuervo, Henar
dc.contributor.authorCrisan, Mihaela
dc.date.accessioned2024-01-17T12:24:31Z
dc.date.available2024-01-17T12:24:31Z
dc.date.issued2022-07-19
dc.description.abstractHematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThe authors would like to thank Elaine Dzierzak and Bruno Pe´ ault for comments on the manuscript and our laboratory members for discussion of our data. We thank James Downing for donating Runx1-IRES-GFP mice and James Ashmore for bioinformatics advice. We are grateful to staff of the University of Edinburgh imaging, flow cytometry, and animal units for their help, Edinburgh Genomics for library sequencing, and Edinburgh Compute and Data Facility for resources provided. We acknowledge the European Hematology Association (EHA) Advance Longterm Fellowship (2882492\R83480), the Academy of Medical Sciences (AMS) Springboard Award (SBF001\1007), the Association Francaise contre la Myopathie (AFM) Trampoline Award (22134/6744359 I190311-2308), the British Heart Foundation (BHF) Research Excellence Award (REA3) pump-priming grant (RE/18/5/34216), and the University of Edinburgh Wellcome Trust Institutional Strategic Support Fund (ISSF3, 199MCR/J22739) to M.C. N.C.H. is supported by a Wellcome Trust Senior Research Fellowship in Clinical Science (219542/Z/19/Z), Medical Research Council (MRC), Chan Zuckerberg Initiative Seed Network Grant, and British Heart Foundation (BHF) grants (RM/17/3/33381 and RE/18/5/ 34216). C.B. is supported by Swedish Research Council (2015-00550), Swedish Cancer Society (150735), and Knut and Alice Wallenberg Foundation (2015.0030).es_ES
dc.format.number3es_ES
dc.format.page111114es_ES
dc.format.volume40es_ES
dc.identifier.citationCell Rep. 2022 Jul 19;40(3):111114.es_ES
dc.identifier.doi10.1016/j.celrep.2022.111114es_ES
dc.identifier.e-issn2211-1247es_ES
dc.identifier.journalCell reportses_ES
dc.identifier.pubmedID35858557es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17202
dc.language.isoenges_ES
dc.publisherCell Presses_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genética Molecular de la Angiogénesises_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshMesonephroses_ES
dc.subject.meshZebrafishes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshHematopoiesises_ES
dc.subject.meshHematopoietic Stem Cellses_ES
dc.subject.meshMicees_ES
dc.subject.meshReceptor, Platelet-Derived Growth Factor betaes_ES
dc.subject.meshStromal Cellses_ES
dc.titlePDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication

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