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Protocol for the assessment of mTOR activity in mouse primary hepatocytes.

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dc.contributor.authorPlata-Gómez, Ana Belén
dc.contributor.authorCrespo, María
dc.contributor.authorde la Calle Arregui, Celia
dc.contributor.authorde Prado-Rivas, Lucía
dc.contributor.authorSabio, Guadalupe
dc.contributor.authorEfeyan, Alejo
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)es_ES
dc.date.accessioned2024-03-11T12:06:07Z
dc.date.available2024-03-11T12:06:07Z
dc.date.issued2021-12-17
dc.description.abstractWe present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021).es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe thank CNIO Animal Facility for excellent technical support. Research was supported by the RETOS Projects Program of the Spanish Ministry of Science, Innovation and Universities, the Spanish State Research Agency (AEI/10.13039/ 501100011033) co-funded by the European Regional Development Fund (SAF2015-67538-R and PID2019-104012RB-I00), the EU-H2020 Program (ERC-2014-STG638891), an Excellence Network Grant from MICIU/AEI (SAF2016-81975-REDT), a Ramon y Cajal Award from MICIU/AEI (RYC-2013-13546), a SpanishAssociation Against Cancer Research Scientific Foundation laboratory grant (LABAE16001EFEY/AECC), Beca de Investigacio´ n en Oncologı´a Olivia Roddom, a FERO Grant for Research in Oncology (to A.E.), EFSD/Lilly European Diabetes Research Programme, MICIU (PID2019-104399RB-I00), Fundacio´ n AECC PROYE19047SABI, and Comunidad de Madrid IMMUNOTHERCAN-CM B2017/BMD-3733 (to G.S.). A.E. and G.S. are EMBO Young Investigators. A.B.P.-G., M.C., C.d.l.C.A., and L.d.P.-R. are recipients of Ayudas de contratos predoctorales para la formacio´ n de doctores from MICIU/AEI (BES-2017-081381, BES-2017-079711, BES-2015-073776, and PRE-2019-090891).es_ES
dc.format.number4es_ES
dc.format.page100918es_ES
dc.format.volume2es_ES
dc.identifier.citationSTAR Protoc. 2021 Oct 29;2(4):100918.es_ES
dc.identifier.doi10.1016/j.xpro.2021.100918es_ES
dc.identifier.e-issn2666-1667es_ES
dc.identifier.journalSTAR protocolses_ES
dc.identifier.pubmedID34755120es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/18915
dc.language.isoenges_ES
dc.publisherCell Presses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/AEI/10.13039/501100011033es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2015-67538-Res_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-104012RB-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2016-81975-REDTes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/RYC-2013-13546es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BES-2017-081381es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BES-2017-079711es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BES-2015-073776es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PRE-2019-090891es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-104399RB-I00es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/ERC-2014-STG638891es_ES
dc.relation.publisherversion10.1016/j.xpro.2021.100918es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Papel de las quinasas activadas por el estrés en el desarrollo de enfermedades cardiovasculares, diabetes y cánceres_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshTOR Serine-Threonine Kinaseses_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Culture Techniqueses_ES
dc.subject.meshCells, Culturedes_ES
dc.subject.meshHepatocyteses_ES
dc.subject.meshLiveres_ES
dc.subject.meshMicees_ES
dc.subject.meshSignal Transductiones_ES
dc.titleProtocol for the assessment of mTOR activity in mouse primary hepatocytes.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication7de1300f-8563-434d-b693-41b7c8c6fdd1
relation.isAuthorOfPublicationfa895eb1-39d5-447c-9b8c-314f35de09c9
relation.isAuthorOfPublication.latestForDiscovery7de1300f-8563-434d-b693-41b7c8c6fdd1

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