Publication:
USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy.

Simple item page
dc.contributor.authorCampos-Iglesias, Diana
dc.contributor.authorFraile, Julia M
dc.contributor.authorBretones, Gabriel
dc.contributor.authorMontero, Alejandro A
dc.contributor.authorBonzon-Kulichenko, Elena
dc.contributor.authorVazquez, Jesus
dc.contributor.authorLópez-Otín, Carlos
dc.contributor.authorFreije, José M P
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderFundación La Caixaes_ES
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)es_ES
dc.contributor.funderFundación Cajastur-Liberbankes_ES
dc.date.accessioned2023-09-15T09:22:06Z
dc.date.available2023-09-15T09:22:06Z
dc.date.issued2023-01-26
dc.description.abstractThe spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe thank A.P. Ugalde, D. Rodríguez, J.G. Pérez-Silva, Y. Español and F. Rodríguez for helpful comments and advice. We also thank S.A. Miranda, D. Álvarez-Puente and C. Garabaya for excellent technical assistance, as well as the staff of the scientific core facilities from the University of Oviedo (Unidad de Ensayos Biotecnológicos y Biomédicos, Servicios Científico-Técnicos). This work was supported by the Ministerio de Ciencia e Innovación (SAF2017-87655-R, PDI2020-118394RB-100 and PGC2018- 097019-B-I00), “la Caixa” Banking Foundation (project code HR17-00247), and the European Research Council (742067, DeAge). The IUOPA is funded by the Asturian Government and Fundación Cajastur-Liberbank.es_ES
dc.format.number1es_ES
dc.format.page60es_ES
dc.format.volume14es_ES
dc.identifier.citationCell Death Dis. 2023 Jan 26;14(1):60.es_ES
dc.identifier.doi10.1038/s41419-023-05600-xes_ES
dc.identifier.e-issn2041-4889es_ES
dc.identifier.journalCell death & diseasees_ES
dc.identifier.pubmedID36702832es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16466
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2017-87655-Res_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PDI2020-118394RB-100es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PGC2018-097019-B-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/HR17-00247es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/ERC/742067es_ES
dc.relation.publisherversion10.1038/s41419-023-05600-xes_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshM Phase Cell Cycle Checkpointses_ES
dc.subject.meshSpindle Apparatuses_ES
dc.subject.meshHumanses_ES
dc.subject.meshAneuploidyes_ES
dc.subject.meshMitosises_ES
dc.subject.meshDeubiquitinating Enzymeses_ES
dc.subject.meshCell Cycle Proteinses_ES
dc.subject.meshUbiquitin Thiolesterasees_ES
dc.titleUSP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationb4bd3b0d-b0ba-4157-aa80-ab71747b6bb9
relation.isAuthorOfPublication9743763b-919c-4fa9-a53c-57c41be5e0ac
relation.isAuthorOfPublication.latestForDiscoveryb4bd3b0d-b0ba-4157-aa80-ab71747b6bb9

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
USP49 deubiquitinase regulates_Cell Death Dis_2023.pdf
Size:
2.35 MB
Format:
Adobe Portable Document Format
Description:
Artículo
Download

Collections

Grupos de investigación