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Detection of chromosome instability by interphase FISH in mouse and human tissues.

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dc.contributor.authorTorres-Ruiz, Raul
dc.contributor.authorGrazioso, Tatiana P
dc.contributor.authorBrandt, Marta
dc.contributor.authorMartinez-Lage, Marta
dc.contributor.authorRodriguez Perales, Sandra
dc.contributor.authorDjouder, Nabil
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderAsociación Española Contra el Cáncer
dc.date.accessioned2024-02-08T14:31:48Z
dc.date.available2024-02-08T14:31:48Z
dc.date.issued2021-09-17
dc.description.abstractChromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis work was funded by grants to N.D. from the State Research Agency (AEI, 10.13039/501100011033) from the Spanish Ministry of Science and Innovation (projects SAF2016-76598-R, SAF2017-92733-EXP, RTI2018-094834-B-I00, and RED2018-102723-T) , cofounded by European Regional Development Fund (ERDF) . S.R-P. is supported by grants from the Spanish National Research and Development Plan, Instituto de Salud Carlos III, and Spanish Ministry of Science and Innovation cofounded by ERDF (PI17/02303; DTS19/00111 and BIO2017-91272-EXP) and by the Asociacion Espanola Contra el Cancer (AECC) . R.T.-R. was supported by a postdoctoral fellowship from the AECC. This work was developed at the CNIO funded by the Health Institute Carlos III (ISCIII) and the Spanish Ministry of Science and Innovation.es_ES
dc.format.number3es_ES
dc.format.page100631es_ES
dc.format.volume2es_ES
dc.identifier.citationSTAR Protoc . 2021;2(3):100631.es_ES
dc.identifier.doi10.1016/j.xpro.2021.100631es_ES
dc.identifier.e-issn2666-1667es_ES
dc.identifier.journalSTAR protocolses_ES
dc.identifier.pubmedID34258592es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17562
dc.language.isoenges_ES
dc.publisherElsevier
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/SAF2016-76598-Res_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/SAF2017-92733-EXPes_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/SAF2017-92733-EXPes_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/RTI2018-094834-B-I0es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.xpro.2021.100631.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Factores de Crecimiento, Nutrientes y Cánceres_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshChromosomal Instabilityes_ES
dc.subject.meshInterphasees_ES
dc.subject.meshAnimalses_ES
dc.subject.meshChromosomes, Artificial, Bacteriales_ES
dc.subject.meshDNAes_ES
dc.subject.meshElectrophoresis, Agar Geles_ES
dc.subject.meshIn Situ Hybridization, Fluorescencees_ES
dc.subject.meshMicees_ES
dc.subject.meshParaffin Embeddinges_ES
dc.titleDetection of chromosome instability by interphase FISH in mouse and human tissues.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
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