Publication:
Cbfa-1 mediates nitric oxide regulation of MMP-13 in osteoblasts.

Simple item page
dc.contributor.authorZaragoza, Carlos
dc.contributor.authorLopez-Rivera, Esther
dc.contributor.authorGarcia-Rama, Concepcion
dc.contributor.authorSaura, Marta
dc.contributor.authorMartinez-Ruiz, Antonio
dc.contributor.authorLizarbe, Tania R
dc.contributor.authorMartín-de-Lara, Fernando
dc.contributor.authorLamas, Santiago
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)
dc.contributor.funderMinisterio de Educación y Ciencia (España)
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderComunidad de Madrid (España)
dc.contributor.funderSociedad Española de Nefrología
dc.contributor.funderCentro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares)
dc.contributor.funderMinisterio de Sanidad y Consumo (España)
dc.date.accessioned2021-02-17T09:02:52Z
dc.date.available2021-02-17T09:02:52Z
dc.date.issued2006-05-01
dc.description.abstractDuring bone development, osteoblast differentiation requires remodeling of the extracellular matrix. Although underlying mechanisms have not been elucidated, evidence points to the participation of the nitric oxide (NO) and cyclic guanosine 3',5'-monophosphate (cGMP) system. Here, we detected increased matrix metalloproteinase (MMP)-13 mRNA, protein and activity, as well as increased inducible NO synthase (iNOS) and NO production during the differentiation of MC3T3-E1 osteoblasts. Transcriptional activity of the MMP-13 promoter was augmented by NO, 8-bromo-cGMP (8-Br-cGMP), and by a dominant-positive form of protein kinase G (PKG1-alpha). The stimulatory effect on the MMP-13 promoter was partially inhibited by mutation of the osteoblast-specific element 2 (OSE-2) binding site. Core binding factor-1 (Cbfa-1) expression peaked at 7 days of differentiation, and was phosphorylated by PKG in vitro. Cbfa-1 was localized to cell nuclei, and its translocation was inhibited by the iNOS inhibitor 1400W. Immunohistological examination revealed that MMP-13 and Cbfa-1 expression levels are both reduced in 17-day-old embryos of iNOS-deficient mice. Silencing of Cbfa-1 mRNA blocked MMP-13 expression without interfering with endogenous NO production, confirming its role in NO-induced MMP-13 expression by MC3T3-E1 cells. The results described here suggest a mechanism by which NO regulates osteogenesis.es_ES
dc.description.peerreviewedSíes_ES
dc.format.numberPt 9es_ES
dc.format.page1896-902es_ES
dc.format.volume119es_ES
dc.identifier.citationJ Cell Sci. 2006; 119(Pt 9):1896-902es_ES
dc.identifier.doi10.1242/jcs.02895es_ES
dc.identifier.issn0021-9533
dc.identifier.journalJournal of cell sciencees_ES
dc.identifier.pubmedID16636074es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11919
dc.language.isoenges_ES
dc.publisherThe Company of Biologistses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2002-00399es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2005- 06025es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/2FD97-1432es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF 2000-0149es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2003- 01039es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/C03/01es_ES
dc.relation.publisherversionhttps://doi.org/10.1242/jcs.02895es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Antiguos CNICes_ES
dc.rights.accessRightsopen accesses_ES
dc.subject.meshGene Expression Regulation, Enzymologices_ES
dc.subject.mesh3T3 Cellses_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Differentiationes_ES
dc.subject.meshCore Binding Factor Alpha 1 Subunites_ES
dc.subject.meshCyclic GMPes_ES
dc.subject.meshCyclic GMP-Dependent Protein Kinaseses_ES
dc.subject.meshEmbryo, Mammalianes_ES
dc.subject.meshGene Silencinges_ES
dc.subject.meshHumanses_ES
dc.subject.meshMatrix Metalloproteinase 13es_ES
dc.subject.meshMicees_ES
dc.subject.meshMice, Inbred C57BLes_ES
dc.subject.meshMice, Knockoutes_ES
dc.subject.meshNitric Oxidees_ES
dc.subject.meshNitric Oxide Synthase Type IIes_ES
dc.subject.meshOsteoblastses_ES
dc.subject.meshOsteogenesises_ES
dc.subject.meshRNA, Messengeres_ES
dc.subject.meshSecond Messenger Systemses_ES
dc.subject.meshTranscription, Genetices_ES
dc.titleCbfa-1 mediates nitric oxide regulation of MMP-13 in osteoblasts.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication02f2e717-5466-43cf-809e-e413036ff4c8
relation.isAuthorOfPublication.latestForDiscovery02f2e717-5466-43cf-809e-e413036ff4c8
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Cbfa-1MediatesNitric_2006.pdf
Size:
640.11 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Grupos de investigación