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Pseudodynamic analysis of heart tube formation in the mouse reveals strong regional variability and early left–right asymmetry

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dc.contributor.authorEsteban, Isaac
dc.contributor.authorSchmidt, Patrick
dc.contributor.authorDesgrange, Audrey
dc.contributor.authorRaiola, Morena
dc.contributor.authorTemiño, Susana
dc.contributor.authorMeilhac, Sigolène M.
dc.contributor.authorKobbelt, Leif
dc.contributor.authorTorres, Miguel
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)es_ES
dc.contributor.funderUnión Europea. Comisión Europea. H2020es_ES
dc.contributor.funderComunidad de Madrid (España)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderGerman Research Foundationes_ES
dc.contributor.funderInstitut Pasteures_ES
dc.contributor.funderFundación ProCNICes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)es_ES
dc.date.accessioned2022-12-01T14:51:42Z
dc.date.available2022-12-01T14:51:42Z
dc.date.issued2022-05-16
dc.description.abstractUnderstanding organ morphogenesis requires a precise geometrical description of the tissues involved in the process. The high morphological variability in mammalian embryos hinders the quantitative analysis of organogenesis. In particular, the study of early heart development in mammals remains a challenging problem due to imaging limitations and complexity. Here, we provide a complete morphological description of mammalian heart tube formation based on detailed imaging of a temporally dense collection of mouse embryonic hearts. We develop strategies for morphometric staging and quantification of local morphological variations between specimens. We identify hot spots of regionalized variability and identify Nodal-controlled left–right asymmetry of the inflow tracts as the earliest signs of organ left–right asymmetry in the mammalian embryo. Finally, we generate a three-dimensional+t digital model that allows co-representation of data from different sources and provides a framework for the computer modeling of heart tube formationes_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe thank members of the Torres laboratory for fruitful discussions and advice on this work. Microscopy was conducted at the Microscopy & Dynamic Imaging,CNIC, ICTSReDib, co-funded by MCIN/AEI /10.13039/501100011033and FEDER Una manera de hacer Europa” (#ICTS-2018-04-CNIC-16). We are grateful to F. Sánchez-Cabo for helpful advice on the statistics. I.E. acknowledges attendance to the Computational Image Analysis (CIAN) course at the MBL, Woods Hole, MA (funded by NIH grant R25 GM103792). We thank S. Bernheim, T. Holm Bønnelykke, L. Guillemot and M. Franco for technical assistance and the Imaging platform and the LEAT animal facility of the SFR Necker. The following provided funding for this study: grant PGC2018-096486- B-I00 from the Spanish Ministerio de Ciencia e Innovación to M.T., grant H2020-MSCAITN-2016-722427 from the EU Horizon 2020 program to M.T., S2017/BMD-3875 from the Comunidad de Madrid to M.T., predoctoral fellowship BFU2015-71519-P from the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to I.E., partial funding from the German Research Foundation within the Gottfried Wilhelm Leibniz program the Excellence Strategy of the Federal Government and the Länder to L.K., support from the core funding from the Institut Pasteur to S.M.M. and state fundings from the Agence Nationale de la Recherche under the ‘Investissements d’avenir’ program (ANR-10-IAHU-01) to S.M.M. S.M.M. is an INSERM research director. The CNIC is supported by the Ministerio de Ciencia e Innovación and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S).es_ES
dc.format.page504–517es_ES
dc.format.volume1es_ES
dc.identifier.citationEsteban, I., Schmidt, P., Desgrange, A. et al. Pseudodynamic analysis of heart tube formation in the mouse reveals strong regional variability and early left–right asymmetry. Nat Cardiovasc Res 1, 504–517 (2022)es_ES
dc.identifier.doidoi.org/10.1038/s44161-022-00065-1es_ES
dc.identifier.e-issn2731-0590es_ES
dc.identifier.journalNature Cardiovascular Researches_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/15250
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/10.13039/501100011033es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PGC2018-096486-B-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BFU2015-71519-Pes_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/CEX2020-001041-Ses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/H2020-MSCAITN-2016-722427es_ES
dc.relation.publisherversiondoi.org/10.1038/s44161-022-00065-es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Control Genético del Desarrollo y Regeneración de Órganoses_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titlePseudodynamic analysis of heart tube formation in the mouse reveals strong regional variability and early left–right asymmetryes_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationbc154711-df10-4239-9eef-4b7b1c877ade
relation.isAuthorOfPublication6ec1130e-9194-41d3-b53f-eba5fc1af5c9
relation.isAuthorOfPublication.latestForDiscoverybc154711-df10-4239-9eef-4b7b1c877ade

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