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Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.

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dc.contributor.authorArévalo, Beatriz
dc.contributor.authorBlázquez-García, Marina
dc.contributor.authorValverde, Alejandro
dc.contributor.authorSerafín, Verónica
dc.contributor.authorMontero-Calle, Ana Maria
dc.contributor.authorSolis-Fernandez, Guillermo
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorCampuzano, Susana
dc.contributor.authorYáñez-Sedeño, Paloma
dc.contributor.authorPingarrón, José M
dc.contributor.funderMinisterio de Ciencia y Universidades (España)
dc.contributor.funderMinisterio de Economía e Innovación (España)
dc.contributor.funderComunidad de Madrid (España)
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderMinisterio de Educación, Cultura y Deporte (España)
dc.contributor.funderResearch Foundation - Flanders
dc.contributor.funderConferencia de Rectores de las Universidades Españolas
dc.date.accessioned2022-04-20T09:56:35Z
dc.date.available2022-04-20T09:56:35Z
dc.date.issued2022-03-14
dc.description.abstractA dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS2/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL-1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Financial support was provided by Spanish Ministerio de Ciencia, Innovacion y Universidades (RTI2018-096,135-B-I00), Spanish Ministerio de Ciencia e Innovación (PID2019-103899RB-I00) Research Project, and the TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid (Grant S2018/NMT-4349). Financial support of the PI17CIII/00045 and PI20CIII/00019 grants from the AES-ISCIII program was also provided to R. B. A predoctoral contract from the Spanish Ministerio de Ciencia, Innovacion y Universidades (PRE2019-087,596) was provided to B. Arévalo. A FPU predoctoral contract supported by the Spanish Ministerio de Educación, Cultura y Deporte was provided to A. M.-C. G. S.-F. is recipient of a predoctoral contract (grant number 1193818 N) supported by The Flanders Research Foundation (FWO).es_ES
dc.format.number4es_ES
dc.format.page143es_ES
dc.format.volume189es_ES
dc.identifier.citationMikrochim Acta. 2022;189(4):143.es_ES
dc.identifier.doi10.1007/s00604-022-05250-4es_ES
dc.identifier.e-issn1436-5073es_ES
dc.identifier.journalMikrochimica Actaes_ES
dc.identifier.pubmedID35286499es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14131
dc.language.isoenges_ES
dc.publisherSpringer
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/RTI2018-096,135-B-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-103899RB-I00es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PRE2019-087,596es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/1193818N
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/PI17CIII/00045es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/PI20CIII/00019es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/s00604-022-05250-4es_ES
dc.repisalud.centroISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC)es_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAPRILes_ES
dc.subjectAmperometryes_ES
dc.subjectBAFFes_ES
dc.subjectCRCes_ES
dc.subjectDual immunoplatformes_ES
dc.subjectMoS2/MWCNTses_ES
dc.subjectSLEes_ES
dc.subjectSerumes_ES
dc.titleBinary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.es_ES
dc.typeresearch articlees_ES
dc.type.hasVersionVoRes_ES
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