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Cohesin organizes chromatin loops at DNA replication factories.

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dc.contributor.authorGuillou, Emmanuelle
dc.contributor.authorIbarra, Arkaitz
dc.contributor.authorCoulon, Vincent
dc.contributor.authorCasado-Vela, Juan
dc.contributor.authorRico, Daniel
dc.contributor.authorCasal, Ignacio
dc.contributor.authorSchwob, Etienne
dc.contributor.authorLosada, Ana
dc.contributor.authorMendez, Juan
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderFrench National Cancer Institute
dc.contributor.funderFundación Caja Madrid
dc.contributor.funderFondation pour la recherche médicale (Francia)
dc.contributor.funderBasque Government (España)
dc.date.accessioned2024-02-08T19:23:24Z
dc.date.available2024-02-08T19:23:24Z
dc.date.issued2010-12-15
dc.description.abstractGenomic DNA is packed in chromatin fibers organized in higher-order structures within the interphase nucleus. One level of organization involves the formation of chromatin loops that may provide a favorable environment to processes such as DNA replication, transcription, and repair. However, little is known about the mechanistic basis of this structuration. Here we demonstrate that cohesin participates in the spatial organization of DNA replication factories in human cells. Cohesin is enriched at replication origins and interacts with prereplication complex proteins. Down-regulation of cohesin slows down S-phase progression by limiting the number of active origins and increasing the length of chromatin loops that correspond with replicon units. These results give a new dimension to the role of cohesin in the architectural organization of interphase chromatin, by showing its participation in DNA replication.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe thank D. Megias, the CNIO Confocal Microscopy Unit, L. Martinez, A. Garcia, and the CNIO Flow Cytometry Unit for excellent technical assistance; M. Drac and T. Gostan of the Montpellier DNA-combing facility for surface preparation and automated image analysis; A. Cuadrado for the RT-PCR analyses of CDC6 expression levels; K. Shirahige, J.M. Peters, and G. Legube for providing the genomic coordinates of CBSs; M. E. Terret for the protocol for DNA fiber preparation; R. Freire for the gift of Chk1 siRNA; members of the Chromosome Dynamics and DNA Replication groups for many discussions; and O. Fernandez-Capetillo, M. Lemaire, and M. Serrano for comments on the manuscript. This work was supported by the Spanish Ministry of Science and Innovation (J.M., A. L., and I. C.), Consolider Ingenio 2010 and Fundacion Caja Madrid (J.M. and A. L.), Comunidad de Madrid (I. C.), and Institut National du Cancer and Association pour la Recherche sur le Cancer (E. S.). E. G. and V. C. were supported by the Fondation pour la Recherche Medicale and Institut National du Cancer, respectively. A. I. was supported by a fellowship from the Basque Governmentes_ES
dc.format.number24es_ES
dc.format.page2812es_ES
dc.format.volume24es_ES
dc.identifier.citationGenes Dev . 2010 ;24(24):2812-22es_ES
dc.identifier.doi10.1101/gad.608210es_ES
dc.identifier.e-issn1549-5477es_ES
dc.identifier.journalGenes & developmentes_ES
dc.identifier.pubmedID21159821es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17660
dc.language.isoenges_ES
dc.publisherCold Spring Harbor Laboratory Press
dc.relation.publisherversionhttps://doi.org/10.1101/gad.608210.es_ES
dc.repisalud.institucionISCIIIes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Dinámica Cromosómicaes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshDNA Packaginges_ES
dc.subject.meshDNA Replicationes_ES
dc.subject.meshCell Cycle Proteinses_ES
dc.subject.meshCell Linees_ES
dc.subject.meshChromatines_ES
dc.subject.meshChromosomal Proteins, Non-Histonees_ES
dc.subject.meshHumanses_ES
dc.subject.meshInterphasees_ES
dc.subject.meshReplication Origines_ES
dc.subject.meshS Phasees_ES
dc.subject.meshCohesinses_ES
dc.titleCohesin organizes chromatin loops at DNA replication factories.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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