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Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

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dc.contributor.authorAndres-Manzano, Maria J.
dc.contributor.authorAndres, Vicente
dc.contributor.authorDorado, Beatriz
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderUnión Europea. Comisión Europea
dc.contributor.funderProgeria Research Foundation
dc.contributor.funderFundación ProCNIC
dc.date.accessioned2019-07-19T05:57:31Z
dc.date.available2019-07-19T05:57:31Z
dc.date.issued2015
dc.description.abstractMethods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.es_ES
dc.description.sponsorshipThe authors thank Simon Bartlett for editorial assistance. Work in VA’s laboratory is supported by grants SAF2013-46663-R and RD12/0042/0028 from the Spanish Ministerio de Economía y Competitividad (MINECO) with co-funding from the Fondo Europeo de Desarrollo Regional (FEDER), the European Commission (Liphos, Grant Agreement No. 317916), and the Progeria Research Foundation (Established Investigator Award). The CNIC is supported by the MINECO and the Pro-CNIC Foundation.es_ES
dc.format.page99
dc.format.volume1339
dc.identifier.citationMethods Mol Biol. 2015; 1339:85-99es_ES
dc.identifier.doi10.1007/978-1-4939-2929-0_5es_ES
dc.identifier.e-issn1940-6029
dc.identifier.isbn978-1-4939-2929-0es_ES
dc.identifier.issn1064-3745
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)
dc.identifier.pubmedID26445782
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7948
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.ispartofseriesMethods in Molelcular Biologyes_ES
dc.relation.ispartofseriesnumber1339es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-46663-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/0028es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317916/EUes_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-4939-2929-0_5es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genéticaes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAortaes_ES
dc.subjectAortic rootes_ES
dc.subjectAtherosclerosises_ES
dc.subjectDissectiones_ES
dc.subjectHematoxylin-eosines_ES
dc.subjectOil Red Oes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshAortaes_ES
dc.subject.meshAortic Diseaseses_ES
dc.subject.meshAtherosclerosises_ES
dc.subject.meshDisease Models, Animales_ES
dc.subject.meshDissectiones_ES
dc.subject.meshImage Interpretation, Computer-Assistedes_ES
dc.subject.meshMicees_ES
dc.subject.meshMicroscopyes_ES
dc.subject.meshParaffin Embeddinges_ES
dc.subject.meshPlaque, Atherosclerotices_ES
dc.subject.meshSeverity of Illness Indexes_ES
dc.titleOil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Rootes_ES
dc.typebook partes_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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relation.isAuthorOfPublication3bb85851-071a-490a-976b-c234983847a7
relation.isAuthorOfPublication6355de6c-f1f5-451f-a89f-9bcc764f6852
relation.isAuthorOfPublication.latestForDiscoveryb3e1effb-8f0e-4272-be7d-50c5f8c9d516

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