Publication:
The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.

Simple item page
dc.contributor.authorAparicio, Tomás
dc.contributor.authorGuillou, Emmanuelle
dc.contributor.authorColoma, Javier
dc.contributor.authorMontoya, Guillermo
dc.contributor.authorMéndez, Juan
dc.date.accessioned2024-02-08T20:02:43Z
dc.date.available2024-02-08T20:02:43Z
dc.date.issued2009-04
dc.description.abstractThe GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45-MCM-GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1-S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipSpanish Ministry of Science and Innovation (BFU-04886, CSD2007-0015 and a 'Ramon y Cajal' contract to J. M., BFU2008-01344 to G. M., PhD studentships to T. A. and J. C.); European Union ( MC IRG FP6-031129 to J. M., 3D-Repertoire LSHG-CT-2005-512028 to G. M.); Fundacion Caja Madrid; postdoctoral fellowship from the Fondation Recherche Medicale, France ( to E. G.). Funding for open access charge: Spanish Ministry of Science and Innovation.es_ES
dc.format.number7es_ES
dc.format.page2087es_ES
dc.format.volume37es_ES
dc.identifier.citationNucleic Acids Res . 2009;37(7):2087-95es_ES
dc.identifier.doi10.1093/nar/gkp065es_ES
dc.identifier.e-issn1362-4962es_ES
dc.identifier.journalNucleic acids researches_ES
dc.identifier.pubmedID19223333es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17663
dc.language.isoenges_ES
dc.publisherOxford University Press
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/BFU-04886es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/CSD2007-0015es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/BFU2008-01344es_ES
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkp065.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Replicación de ADNes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshDNA Replicationes_ES
dc.subject.meshAntibodieses_ES
dc.subject.meshCell Cycle Proteinses_ES
dc.subject.meshCell Linees_ES
dc.subject.meshCell Proliferationes_ES
dc.subject.meshChromatines_ES
dc.subject.meshChromosomal Proteins, Non-Histonees_ES
dc.subject.meshHumanses_ES
dc.subject.meshProtein Subunitses_ES
dc.subject.meshS Phasees_ES
dc.titleThe human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationf1fb14ab-d2a5-476e-b0fa-9b6c89d7cb11
relation.isAuthorOfPublication.latestForDiscoveryf1fb14ab-d2a5-476e-b0fa-9b6c89d7cb11
relation.isPublisherOfPublication465a0b1e-d9df-4342-b738-86ffcafc4bcf
relation.isPublisherOfPublication.latestForDiscovery465a0b1e-d9df-4342-b738-86ffcafc4bcf

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
ThehumanGINScomple_2009.pdf
Size:
7.9 MB
Format:
Adobe Portable Document Format
Description:
Artículo principal
Download

Collections

Grupos de investigación