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Use of a LiESP/QA-21 vaccine (CaniLeish) stimulates an appropriate Th1-dominated cell-mediated immune response in dogs

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dc.contributor.authorMoreno, Javier
dc.contributor.authorVouldoukis, Ioannis
dc.contributor.authorMartin, Virginie
dc.contributor.authorMcGahie, David
dc.contributor.authorCuisinier, Anne-Marie
dc.contributor.authorGueguen, Sylvie
dc.date.accessioned2018-12-27T11:41:36Z
dc.date.available2018-12-27T11:41:36Z
dc.date.issued2012-06-19
dc.description.abstractCanine leishmaniasis is an important zoonotic disease of dogs. The clinical outcome of infection is variable, with the efficiency of the immune response being the key determining factor. There is now a general consensus that a predominant Th1 immune profile in an overall mixed Th1/Th2 response is associated with resistance in dogs, and the absence of a strong Th1 influence is associated with a progression to clinical disease. As a result, there has been a growing demand for vaccines that can induce a specific, strong Th1 response. In this study, we measured the impact of a primary course of a newly available LiESP/QA-21 vaccine on selected humoral and cellular markers of the canine immune response during the onset of immunity. All vaccinated dogs developed a humoral response characterised by IgG2 production. More importantly, vaccinated dogs developed significantly stronger cell-mediated immunity responses than did control dogs. Vaccination induced specific cellular reactivity to soluble Leishmania antigens, with a Leishmania-specific lymphoproliferation (p = 0.0072), characterised by an increased population of T lymphocytes producing IFN-γ (p = 0.0021) and a significant ability of macrophages to reduce intracellular parasite burdens in vitro after co-culture with autologous lymphocytes (p = 0.0014). These responses were correlated with induction of the NOS pathway and production of NO derivatives, which has been shown to be an important leishmanicidal mechanism. These results confirm that vaccination with LiESP/QA-21 induces an appropriate Th1-profile cell-mediated response within three weeks of completing the primary course, and that this response effectively reduces the parasite load in pre-infected macrophages in vitro.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipSeveral of the authors are employees of Virbac. Virbac played a direct role in the study design, data collection and analysis, decision to publish and preparation of the manuscriptes_ES
dc.format.number6es_ES
dc.format.pagee1683es_ES
dc.format.volume6es_ES
dc.identifier.citationPLoS Negl Trop Dis. 2012;6(6):e1683es_ES
dc.identifier.doi10.1371/journal.pntd.0001683es_ES
dc.identifier.e-issn1935-2735es_ES
dc.identifier.issn1935-2735es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.identifier.pubmedID22724031es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6967
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0001683es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimalses_ES
dc.subject.meshAntibodies, Protozoanes_ES
dc.titleUse of a LiESP/QA-21 vaccine (CaniLeish) stimulates an appropriate Th1-dominated cell-mediated immune response in dogses_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication831dbac1-fcb6-444a-90e1-4b562eecb934
relation.isAuthorOfPublication.latestForDiscovery831dbac1-fcb6-444a-90e1-4b562eecb934
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