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Glycolytic enzymes can modulate cellular life span.

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dc.contributor.authorKondoh, Hiroshi
dc.contributor.authorLleonart, Matilde E
dc.contributor.authorGil, Jesús
dc.contributor.authorWang, Jing
dc.contributor.authorDegan, Paolo
dc.contributor.authorPeters, Gordon
dc.contributor.authorMartinez Garcia, Maria Dolores
dc.contributor.authorCarnero, Amancio
dc.contributor.authorBeach, David
dc.contributor.funderWellcome Trust
dc.date.accessioned2024-02-01T10:59:28Z
dc.date.available2024-02-01T10:59:28Z
dc.date.issued2005-01-01
dc.descriptionGrant support: Wellcome Trust, Medical Research Council, Cancer Research UK,the Marie Curie and the Hugh and Catherine Stevenson Funds, a Marie Curie fellowship (M.E. Lleonart), and a Human Frontiers Science Program fellowship (J. Gil).The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. We thank Drs. Kevin Brindle and K. Uchida for the human phosphofructokinase clone and antibodies, respectively, Dr. H. Arakawa for p53RFP clone, and Dr. Austin Smith and Robin Lovell-Badge for mouse embryonic stem cells. We are also grateful to all the members of David Beach’s laboratory for helpful advice and discussions.es_ES
dc.description.abstractAn unbiased screen for genes that can immortalize mouse embryonic fibroblasts identified the glycolytic enzyme phosphoglycerate mutase (PGM). A 2-fold increase in PGM activity enhances glycolytic flux, allows indefinite proliferation, and renders cells resistant to ras-induced arrest. Glucosephosphate isomerase, another glycolytic enzyme, displays similar activity and, conversely, depletion of PGM or glucosephosphate isomerase with short interfering RNA triggers premature senescence. Immortalized mouse embryonic fibroblasts and mouse embryonic stem cells display higher glycolytic flux and more resistance to oxidative damage than senescent cells. Because wild-type p53 down-regulates PGM, mutation of p53 can facilitate immortalization via effects on PGM levels and glycolysis.es_ES
dc.description.peerreviewedSíes_ES
dc.format.number1es_ES
dc.format.page177es_ES
dc.format.volume65es_ES
dc.identifier.citationCancer Res . 2005;65(1):177-85.es_ES
dc.identifier.doi10.1158/0008-5472.177.65.1es_ES
dc.identifier.issn0008-5472es_ES
dc.identifier.journalCancer researches_ES
dc.identifier.pubmedID15665293es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17406
dc.language.isoenges_ES
dc.publisherAmerican Association for Cancer Research (AACR)
dc.relation.publisherversionhttps://doi.org/10.1158/0008-5472.177.65.1es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Citometría de Flujoes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshGlycolysises_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Divisiones_ES
dc.subject.meshCell Survivales_ES
dc.subject.meshCells, Culturedes_ES
dc.subject.meshEmbryo, Mammalianes_ES
dc.subject.meshFibroblastses_ES
dc.subject.meshGenes, rases_ES
dc.subject.meshMicees_ES
dc.subject.meshMice, Inbred C57BLes_ES
dc.subject.meshPhosphoglycerate Mutasees_ES
dc.titleGlycolytic enzymes can modulate cellular life span.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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