Publication: Inactivation of Capicua in adult mice causes T-cell lymphoblastic lymphoma
dc.contributor.author | Simón-Carrasco, Lucía | |
dc.contributor.author | Graña Castro, Osvaldo | |
dc.contributor.author | Salmón, Marina | |
dc.contributor.author | Jacob, Harrys K C | |
dc.contributor.author | Gutierrez, Alejandro | |
dc.contributor.author | Jiménez, Gerardo | |
dc.contributor.author | Drosten, Matthias | |
dc.contributor.author | Barbacid, Mariano | |
dc.contributor.funder | Fundación La Marató TV3 | |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Asociación Española Contra el Cáncer | |
dc.contributor.funder | Fundación AXA | |
dc.date.accessioned | 2019-10-01T10:52:20Z | |
dc.date.available | 2019-10-01T10:52:20Z | |
dc.date.issued | 2017-07-15 | |
dc.description.abstract | CIC (also known as Capicua) is a transcriptional repressor negatively regulated by RAS/MAPK signaling. Whereas the functions of Cic have been well characterized in Drosophila, little is known about its role in mammals. CIC is inactivated in a variety of human tumors and has been implicated recently in the promotion of lung metastases. Here, we describe a mouse model in which we inactivated Cic by selectively disabling its DNA-binding activity, a mutation that causes derepression of its target genes. Germline Cic inactivation causes perinatal lethality due to lung differentiation defects. However, its systemic inactivation in adult mice induces T-cell acute lymphoblastic lymphoma (T-ALL), a tumor type known to carry CIC mutations, albeit with low incidence. Cic inactivation in mice induces T-ALL by a mechanism involving derepression of its well-known target, Etv4 Importantly, human T-ALL also relies on ETV4 expression for maintaining its oncogenic phenotype. Moreover, Cic inactivation renders T-ALL insensitive to MEK inhibitors in both mouse and human cell lines. Finally, we show that Ras-induced mouse T-ALL as well as human T-ALL carrying mutations in the RAS/MAPK pathway display a genetic signature indicative of Cic inactivation. These observations illustrate that CIC inactivation plays a key role in this human malignancy. | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.description.sponsorship | We are grateful to Carol MacKintosh (University of Dundee, UK) for the pcDNA5/FRT/TO-GFP-CIC plasmid, and Huda Zoghbi (Baylor College of Medicine, Houston, TX) and Yoontae Lee (University of Pohang, Korea) for Cic antisera. We thank Scott Brown and Robert Holt (University of Vancouver, Canada) for their help with TCR abundance calculations. We also thank Carmen G. Lechuga, Marta San Roman, Raquel Villar, Beatriz Jimenez, and Nuria Cabrera for excellent technical assistance. We value the support of Sagrario Ortega (Transgenic Mice Core Unit, CNIO) for help in generating the Cic mutant mice, Orlando Dominguez (Genomics Core Unit, CNIO) for the RNA-seq analysis, and the Histopathology Core Unit. This work was supported by grants from the Fundacio La Marato de TV3 (20131730/1) to G.J. and M.B., and the European Research Council (ERC-AG/250297-RAS AHEAD), the EU-Framework Programme (HEALTH-F2-2010-259770/LUNGTARGET and HEALTH-2010-260791/EUROCANPLATFORM), the Spanish Ministry of Economy and Competitiveness (SAF2014-59864-R), the Autonomous Community of Madrid (S2011/BDM-2470/ONCOCYCLE), and the Asociacion Espanola contra el Cancer (AECC) (GC16173694BARB) to M.B. M.B. is the recipient of an Endowed Chair from the AXA Research Fund. L.S.-C. was supported by a fellowship from the Programa de Formacion de Personal Investigator (FPI) of the Spanish Ministry of Economy and Competitiveness. M.D. and M.B. conceived and designed the study. L.S.-C., O.G., G.J., M.D., and M.B. developed the methodology. L.S.-C., O.G., M.S., and M.D. acquired the data. L.S.-C., O.G., M.S., H.K.C.J., G.J., M.D., and M.B. analyzed and interpreted the data. L.S-C., O.G., G.J., M.D., and M.B. wrote, reviewed, and/or revised the manuscript. G.J. provided material support. A. G. analyzed the T-ALL sequencing. M.D. and M.B. supervised the study. | es_ES |
dc.format.number | 14 | es_ES |
dc.format.page | 1456-1468 | es_ES |
dc.format.volume | 31 | es_ES |
dc.identifier.citation | Genes Dev. 2017 ;31(14):1456-1468. | es_ES |
dc.identifier.doi | 10.1101/gad.300244.117 | es_ES |
dc.identifier.e-issn | 1549-5477 | es_ES |
dc.identifier.issn | 0890-9369 | es_ES |
dc.identifier.journal | Genes & development | es_ES |
dc.identifier.pubmedID | 28827401 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/8392 | |
dc.language.iso | eng | es_ES |
dc.publisher | Cold Spring Harbor Laboratory Press | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/EC/250297 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/SAF2014-59864-R | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/S2011/BDM-2470/ONCOCYCLE | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/GC16173694BARB | es_ES |
dc.relation.publisherversion | https://doi.org/ 10.1101/gad.300244.117 | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Oncología Experimental | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | CIC | es_ES |
dc.subject | Etv4 | es_ES |
dc.subject | Ras signaling | es_ES |
dc.subject | T-ALL | es_ES |
dc.subject | Mouse models | es_ES |
dc.subject.mesh | Adenovirus E1A Proteins | es_ES |
dc.subject.mesh | Alleles | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Brain Neoplasms | es_ES |
dc.subject.mesh | Cell Line, Tumor | es_ES |
dc.subject.mesh | Embryonic Development | es_ES |
dc.subject.mesh | Fibroblasts | es_ES |
dc.subject.mesh | Genes, ras | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | MAP Kinase Signaling System | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mutation | es_ES |
dc.subject.mesh | Oligodendroglioma | es_ES |
dc.subject.mesh | Precursor T-Cell Lymphoblastic Leukemia-Lymphoma | es_ES |
dc.subject.mesh | Proto-Oncogene Proteins | es_ES |
dc.subject.mesh | Proto-Oncogene Proteins c-ets | es_ES |
dc.subject.mesh | Repressor Proteins | es_ES |
dc.subject.mesh | Transcription, Genetic | es_ES |
dc.title | Inactivation of Capicua in adult mice causes T-cell lymphoblastic lymphoma | es_ES |
dc.type | journal article | es_ES |
dc.type.hasVersion | VoR | es_ES |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 985e5671-0ac7-4e86-98c2-31a5ffe60751 | |
relation.isAuthorOfPublication | 3064b65c-f19a-4726-a7c1-a4d1664329be | |
relation.isAuthorOfPublication | 728b1f96-276b-4ab5-8640-8964fb72939f | |
relation.isAuthorOfPublication.latestForDiscovery | 985e5671-0ac7-4e86-98c2-31a5ffe60751 |
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