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Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.

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dc.contributor.authorGuadix, Juan Antonio
dc.contributor.authorOrlova, Valeria V
dc.contributor.authorGiacomelli, Elisa
dc.contributor.authorBellin, Milena
dc.contributor.authorRibeiro, Marcelo C
dc.contributor.authorMummery, Christine L
dc.contributor.authorPérez-Pomares, José M
dc.contributor.authorPassier, Robert
dc.date.accessioned2024-01-23T20:13:19Z
dc.date.available2024-01-23T20:13:19Z
dc.date.issued2017-11-22
dc.description.abstractHuman pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.
dc.format.number6es_ES
dc.format.page1754-1764es_ES
dc.format.volume9es_ES
dc.identifier.doi10.1016/j.stemcr.2017.10.023
dc.identifier.e-issn2213-6711es_ES
dc.identifier.journalStem cell reportses_ES
dc.identifier.otherhttp://hdl.handle.net/10668/11845
dc.identifier.pubmedID29173898es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17347
dc.language.isoeng
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectcardiovascular
dc.subjectdifferentiation
dc.subjecthuman pluripotent stem cells
dc.subjectproepicardium
dc.subjectprogenitor cells
dc.subject.meshAnimals
dc.subject.meshBone Morphogenetic Protein 4
dc.subject.meshCardiovascular System
dc.subject.meshCell Adhesion
dc.subject.meshCell Culture Techniques
dc.subject.meshCell Differentiation
dc.subject.meshChick Embryo
dc.subject.meshEmbryonic Development
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshHeart
dc.subject.meshHumans
dc.subject.meshInduced Pluripotent Stem Cells
dc.subject.meshMyocardium
dc.subject.meshMyocytes, Cardiac
dc.subject.meshPericardium
dc.subject.meshReceptor, Platelet-Derived Growth Factor alpha
dc.subject.meshStem Cells
dc.subject.meshTretinoin
dc.titleHuman Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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