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Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector

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dc.contributor.authorTorrellas, Annhymariet
dc.contributor.authorFerrer, Elizabeth
dc.contributor.authorCruz, Israel
dc.contributor.authorLima, Héctor de
dc.contributor.authorDelgado, Olinda
dc.contributor.authorRangel, José Carrero
dc.contributor.authorBravo, José Arturo
dc.contributor.authorChicharro, Carmen
dc.contributor.authorLlanes-Acevedo, Ivonne Pamela
dc.contributor.authorMiles, Michael A
dc.contributor.authorFeliciangeli, María Dora
dc.contributor.funderUnión Europea. Comisión Europea
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)
dc.date.accessioned2020-03-25T17:41:04Z
dc.date.available2020-03-25T17:41:04Z
dc.date.issued2018-12-06
dc.description.abstractBACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which causes ACL in the Andean Region of Venezuela, by identifying the Leishmania and the sand fly species involved in human and dog infections. METHODS Thirty-one biopsies from patients in Mérida and Táchira states with suspected ACL were studied by both parasitological tests (cultures and hamster inoculation) and a molecular test [Internal transcribed spacer 1 (ITS1) nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)]. We also conducted a survey to detect Leishmania infection in dogs (Immunifluorescence antibody test and ITS1 nested PCR-RFLP) and sand flies (ITS1 nested PCR-RFLP) from El Carrizal, a highly endemic focus of ACL in Venezuela. FINDINGS Three different Leishmania species were identified in the clinical samples from humans (Leishmania braziliensis, L. guyanensis, and L. mexicana) and dogs (L. guyanensis and L. mexicana). The predominant sand fly species found were those from the Verrucarum group (infected with L. mexicana) and Lutzomyia migonei (infected with L. guyanensis and L. mexicana). MAIN CONCLUSIONS We show that Lu. migonei may be the putative vector in two ACL epidemiological cycles, involving L. guyanensis and L. mexicana. We also report for the first time the presence of L. guyanensis in domestic animals.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipFinancial support: European Commission project “Control strategies for visceral leishmaniasis (VL) and mucocutaneous leishmaniasis (MCL) in South America: applications of molecular epidemiology” (EC contract INCO-CT2005-015407, LeishEpiNetSA) and by the Ministerio de Ciencia y Tecnología (MPPCTI/FONACIT), Project MISIÓN CIENCIA - Leishmaniasis, 2008000911 - 2. * Present address: Foundation for Innovative New Diagnostics, Geneva, Switzerland. † In memoriames_ES
dc.format.number12es_ES
dc.format.pagee180323es_ES
dc.format.volume113es_ES
dc.identifier.citationMem Inst Oswaldo Cruz. 2018 Dec 6;113(12):e180323.es_ES
dc.identifier.doi10.1590/0074-02760180323es_ES
dc.identifier.e-issn1678-8060es_ES
dc.identifier.issn1678-8060es_ES
dc.identifier.journalMemorias do Instituto Oswaldo Cruzes_ES
dc.identifier.pubmedID30540021es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9337
dc.language.isoenges_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/EC contract INCO-CT2005-015407, LeishEpiNetSA)es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/Project MISIÓN CIENCIA - Leishmaniasis, 2008000911 - 2es_ES
dc.relation.publisherversionhttps://doi.org/10.1590/0074-02760180323es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimalses_ES
dc.subject.meshDNA, Ribosomal Spaceres_ES
dc.subject.meshDogses_ES
dc.subject.meshFemalees_ES
dc.subject.meshHumanses_ES
dc.subject.meshInsect Vectorses_ES
dc.subject.meshLeishmania braziliensises_ES
dc.subject.meshLeishmania guyanensises_ES
dc.subject.meshLeishmania mexicanaes_ES
dc.subject.meshLeishmaniasis, Cutaneouses_ES
dc.subject.meshMolecular Typinges_ES
dc.subject.meshPolymerase Chain Reactiones_ES
dc.subject.meshPolymorphism, Restriction Fragment Lengthes_ES
dc.subject.meshPsychodidaees_ES
dc.subject.meshVenezuelaes_ES
dc.titleMolecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vectores_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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relation.isAuthorOfPublication6f88e6f5-6782-4691-8e3a-775581a76358
relation.isAuthorOfPublication9a854aba-a42f-4418-ad59-fb37d799be86
relation.isAuthorOfPublication.latestForDiscovery4ca3b46e-2faa-4a7d-a134-d198c24d7475
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