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Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps.

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dc.contributor.authorBouchery, Tiffany
dc.contributor.authorMoyat, Mati
dc.contributor.authorSotillo, Javier
dc.contributor.authorSilverstein, Solomon
dc.contributor.authorVolpe, Beatrice
dc.contributor.authorCoakley, Gillian
dc.contributor.authorTsourouktsoglou, Theodora-Dorita
dc.contributor.authorBecker, Luke
dc.contributor.authorShah, Kathleen
dc.contributor.authorKulagin, Manuel
dc.contributor.authorGuiet, Romain
dc.contributor.authorCamberis, Mali
dc.contributor.authorSchmidt, Alfonso
dc.contributor.authorSeitz, Arne
dc.contributor.authorGiacomin, Paul
dc.contributor.authorLe Gros, Graham
dc.contributor.authorPapayannopoulos, Venizelos
dc.contributor.authorLoukas, Alex
dc.contributor.authorHarris, Nicola L
dc.contributor.funderSwiss National Science Foundation
dc.contributor.funderNational Health and Medical Research Council (Australia)
dc.date.accessioned2021-04-06T11:26:41Z
dc.date.available2021-04-06T11:26:41Z
dc.date.issued2020
dc.description.abstractHookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infection is unknown. Using a murine hookworm, Nippostrongylus brasiliensis, we observed neutrophils being rapidly recruited and deploying NETs around skin-penetrating larvae. Neutrophils depletion or NET inhibition altered larvae behavior and enhanced the number of adult worms following murine infection. Nevertheless, larvae were able to mitigate the effect of NETs by secreting a deoxyribonuclease (Nb-DNase II) to degrade the DNA backbone. Critically, neutrophils were able to kill larvae in vitro, which was enhanced by neutralizing Nb-DNase II. Homologs of Nb-DNase II are present in other nematodes, including the human hookworm, Necator americanus, which also evaded NETs in vitro. These findings highlight the importance of neutrophils in hookworm infection and a potential conserved mechanism of immune evasion.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis work was supported by the Swiss National Science Foundation ( SNF310030_156517 ), the Health Research Council of New Zealand , and the Marjorie Barclay Trust , New Zealand. N.L.H. is supported by a National Health and Medical Research Council (NHMRC) of Australia SRF-B fellowship.es_ES
dc.format.number2es_ES
dc.format.page277-289.e6es_ES
dc.format.volume27es_ES
dc.identifier.citationCell Host Microbe . 2020 Feb 12;27(2):277-289.e6.es_ES
dc.identifier.doi10.1016/j.chom.2020.01.011es_ES
dc.identifier.e-issn1934-6069es_ES
dc.identifier.journalCell host & microbees_ES
dc.identifier.pubmedID32053791es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12529
dc.language.isoenges_ES
dc.publisherCell Presses_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.chom.2020.01.011es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDNAse IIes_ES
dc.subjectNippostrongylus brasiliensises_ES
dc.subjectHelminthes_ES
dc.subjectHookwormes_ES
dc.subjectImmune-evasiones_ES
dc.subjectNeutrophil extracellular trapses_ES
dc.subjectNeutrophilses_ES
dc.subject.meshImmune Evasiones_ES
dc.subject.meshAncylostomatoideaes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshEndodeoxyribonucleaseses_ES
dc.subject.meshExtracellular Trapses_ES
dc.subject.meshHost-Parasite Interactionses_ES
dc.subject.meshMicees_ES
dc.subject.meshNeutrophilses_ES
dc.subject.meshNippostrongyluses_ES
dc.subject.meshStrongylida Infectionses_ES
dc.titleHookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps.es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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