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Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38

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dc.contributor.authorMaloney, Jenny G
dc.contributor.authorMolokin, Aleksey
dc.contributor.authorSeguí, Raimundo
dc.contributor.authorMaravilla, Pablo
dc.contributor.authorMartínez-Hernández, Fernando
dc.contributor.authorVillalobos, Guiehdani
dc.contributor.authorTsaousis, Anastasios D
dc.contributor.authorGentekaki, Eleni
dc.contributor.authorMuñoz-Antoli, Carla
dc.contributor.authorKlisiowicz, Debora R
dc.contributor.authorOishi, Camila Y
dc.contributor.authorToledo, Rafael
dc.contributor.authorEsteban, J Guillermo
dc.contributor.authorKöster, Pamela Carolina
dc.contributor.authorde Lucio, Aida
dc.contributor.authorDashti, Alejandro
dc.contributor.authorBailo-Barroso, Begoña
dc.contributor.authorCalero-Bernal, Rafael
dc.contributor.authorGonzalez-Barrio, David
dc.contributor.authorCarmena, David
dc.contributor.authorSantín, Mónica
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderUnited States Department of Agriculture. Agricultural Research Service
dc.date.accessioned2023-07-17T10:35:27Z
dc.date.available2023-07-17T10:35:27Z
dc.date.issued2023
dc.description.abstractThree recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis research was funded by USDA-ARS Project No.: 8042-32000-112-00-D and the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain)-Collaborative Research Network on Tropical Diseases (RICET) and the European Regional Development Fund (ERDF), under project RD12/0018/0013. Additional funding was also provided by the ISCIII under project PI16CIII/00024.es_ES
dc.format.number1es_ES
dc.format.page46es_ES
dc.format.volume11es_ES
dc.identifier.citationMicroorganisms. 2023;11(1):46.es_ES
dc.identifier.doi10.3390/microorganisms11010046es_ES
dc.identifier.issn2076-2607es_ES
dc.identifier.journalMicroorganismses_ES
dc.identifier.pubmedID36677338es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16266
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//RD12%2F0018%2F0013/ES/Enfermedades tropicales/es_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/ISCIII 2016 Modalidad Proyectos de Investigacion en Salud Intramurales. (2016)/PI16CIII/00024es_ES
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms11010046es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBlastocystises_ES
dc.subjectB. lapemies_ES
dc.subjectSubtypees_ES
dc.subjectOxford Nanopore MinIONes_ES
dc.subjectssu rRNAes_ES
dc.titleIdentification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38es_ES
dc.typeresearch articlees_ES
dc.type.hasVersionVoRes_ES
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