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CtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cells

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dc.contributor.authorGómez-Cabello, Daniel
dc.contributor.authorCheca-Rodríguez, Cintia
dc.contributor.authorAbad, María
dc.contributor.authorSerrano Marugan, Manuel
dc.contributor.authorHuertas, Pablo
dc.contributor.funderMinisterio de Ciencia y Competitividad (España)
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)
dc.date.accessioned2019-02-27T09:46:19Z
dc.date.available2019-02-27T09:46:19Z
dc.date.issued2017-02-14
dc.description.abstractAcquired genomic instability is one of the major concerns for the clinical use of induced pluripotent stem cells (iPSCs). All reprogramming methods are accompanied by the induction of DNA damage, of which double-strand breaks are the most cytotoxic and mutagenic. Consequently, DNA repair genes seem to be relevant for accurate reprogramming to minimize the impact of such DNA damage. Here, we reveal that reprogramming is associated with high levels of DNA end resection, a critical step in homologous recombination. Moreover, the resection factor CtIP is essential for cell reprogramming and establishment of iPSCs, probably to repair reprogramming-induced DNA damage. Our data reveal a new role for DNA end resection in maintaining genomic stability during cell reprogramming, allowing DNA repair fidelity to be retained in both human and mouse iPSCs. Moreover, we demonstrate that reprogramming in a resection-defective environment has long-term consequences on stem cell self-renewal and differentiation.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe wish to thank the cytometer unit of CABIMER for technical support, Diana Aguilar for critical reading of the manuscript, andVeronica Raker for style corrections. This work was funded by an R + D + I grantfrom the Spanish Ministry of Economyand Compet- itivity (SAF2013-43255-P) and an ERC Starting Grant (DSBRECA).es_ES
dc.format.number2es_ES
dc.format.page432-445es_ES
dc.format.volume8es_ES
dc.identifier.citationStem Cell Reports. 2017;8(2):432-445.es_ES
dc.identifier.doi10.1016/j.stemcr.2016.12.009es_ES
dc.identifier.e-issn2213-6711es_ES
dc.identifier.issn22136711es_ES
dc.identifier.journalStem cell reportses_ES
dc.identifier.pubmedID28065643es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7239
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-43255-P .es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.stemcr.2016.12.009..es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Supresión Tumorales_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDNA resectiones_ES
dc.subjectCell reprogramminges_ES
dc.subjectGenetic instabilityes_ES
dc.subjectiPSCes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCarrier Proteinses_ES
dc.subject.meshCell Cycle Proteinses_ES
dc.subject.meshCell Differentiationes_ES
dc.subject.meshCell Self Renewales_ES
dc.subject.meshCell Survivales_ES
dc.subject.meshCellular Reprogramminges_ES
dc.subject.meshDNA Damagees_ES
dc.subject.meshGenomic Instabilityes_ES
dc.subject.meshHumanses_ES
dc.subject.meshInduced Pluripotent Stem Cellses_ES
dc.subject.meshNuclear Proteinses_ES
dc.subject.meshGenetic Fitnesses_ES
dc.titleCtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cellses_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationda94122b-9881-4447-b2ba-398f96d92593
relation.isAuthorOfPublication.latestForDiscoveryda94122b-9881-4447-b2ba-398f96d92593
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