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Robust, universal biomarker assay to detect senescent cells in biological specimens

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dc.contributor.authorEvangelou, Konstantinos
dc.contributor.authorLougiakis, Nikolaos
dc.contributor.authorRizou, Sophia V
dc.contributor.authorKotsinas, Athanassios
dc.contributor.authorKletsas, Dimitris
dc.contributor.authorMuñoz-Espín, Daniel
dc.contributor.authorKastrinakis, Nikolaos G
dc.contributor.authorPouli, Nicole
dc.contributor.authorMarakos, Panagiotis
dc.contributor.authorTownsend, Paul
dc.contributor.authorSerrano Marugan, Manuel
dc.contributor.authorBartek, Jiri
dc.contributor.authorGorgoulis, Vassilis G
dc.contributor.funderSwedish Research Council for Health, Working Life and Welfare
dc.contributor.funderDanish National Research Foundation
dc.contributor.funderNovo Nordisk Foundation
dc.date.accessioned2019-03-01T10:50:36Z
dc.date.available2019-03-01T10:50:36Z
dc.date.issued2017-02
dc.description.abstractCellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipAcknowledgments The authors would like to acknowledge Dr Ioannis Dotsikas help in performing the HPLC analysis and thank Dr Vassilis Myrianthopoulos for helpful suggestions. The authors’ work is funded by the European Union’s Seventh Framework Programme (project INsPiRE), the Greek GSRT program of Excellence II (Aristeia II), the Danish Cancer Society, the Danish National Research Foundation (DNRF125).es_ES
dc.format.number1es_ES
dc.format.page192-197es_ES
dc.format.volume16es_ES
dc.identifier.citationAging Cell. 2017;16(1):192-197.es_ES
dc.identifier.doi10.1111/acel.12545es_ES
dc.identifier.e-issn1474-9726es_ES
dc.identifier.issn14749718es_ES
dc.identifier.journalAging celles_ES
dc.identifier.pubmedID28165661es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7266
dc.language.isoenges_ES
dc.publisherWiley
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/284460/EUes_ES
dc.relation.publisherversionhttps://doi: 10.1111/acel.12545.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Supresión Tumorales_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSudan Black Bes_ES
dc.subjectAginges_ES
dc.subjectBiotin‐linked compoundses_ES
dc.subjectImmunohistochemistryes_ES
dc.subjectSenescencees_ES
dc.subject.meshAnimalses_ES
dc.subject.meshBiological Assayes_ES
dc.subject.meshBiomarkerses_ES
dc.subject.meshBiotines_ES
dc.subject.meshCell Line, Tumores_ES
dc.subject.meshHumanses_ES
dc.subject.meshMicees_ES
dc.subject.meshCellular Senescencees_ES
dc.titleRobust, universal biomarker assay to detect senescent cells in biological specimenses_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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