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Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications

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dc.contributor.authorAntich-Rosselló, Miquel
dc.contributor.authorMunar-Bestard, Marta
dc.contributor.authorForteza-Genestra, Maria Antónia
dc.contributor.authorCalvo Benito, Javier
dc.contributor.authorGayà, Antoni
dc.contributor.authorMonjo, Marta
dc.contributor.authorRamis, Joana Maria
dc.date.accessioned2024-10-04T13:22:48Z
dc.date.available2024-10-04T13:22:48Z
dc.date.issued2022-07-11
dc.description.abstractGingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.en
dc.description.sponsorshipInstituto de Salud Carlos III, co-funded by the ESF European Social Fundand the ERDF European Regional Development Fund. Direcció General d´Investigació, Conselleria d´Investigació, Govern Balear and the Institut d´Investigació Sanitária de les Illes Balears. The publication cost was supported by LIBERI from Health Research Institute of the Balearic Islands (IdISBa).es_ES
dc.format.number14es_ES
dc.format.page7668es_ES
dc.format.volume23es_ES
dc.identifier.citationAntich-Rosselló M, Munar-Bestard M, Forteza-Genestra MA, Calvo J, Gayà A, Monjo M, et al. Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications. Int J Mol Sci. 2022 Jul 11;23(14):7668.en
dc.identifier.doi10.3390/ijms23147668
dc.identifier.e-issn1422-0067es_ES
dc.identifier.journalInternational journal of molecular scienceses_ES
dc.identifier.otherhttp://hdl.handle.net/20.500.13003/18126
dc.identifier.pubmedID35887008es_ES
dc.identifier.puiL2018307945
dc.identifier.scopus2-s2.0-85135103611
dc.identifier.urihttps://hdl.handle.net/20.500.12105/23431
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.publisherversionhttps://doi.org/10.3390/ijms23147668en
dc.rights.accessRightsopen accessen
dc.rights.licenseAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.decsMateriales Biocompatibles*
dc.subject.decsVesículas Extracelulares*
dc.subject.decsFibroblastos*
dc.subject.decsQueratinocitos*
dc.subject.decsEncía*
dc.subject.meshKeratinocytes*
dc.subject.meshExtracellular Vesicles*
dc.subject.meshFibroblasts*
dc.subject.meshGingiva*
dc.subject.meshBiocompatible Materials*
dc.titleEvaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applicationsen
dc.typeresearch articleen
dspace.entity.typePublication
relation.isPublisherOfPublication30293a55-0e53-431f-ae8c-14ab01127be9
relation.isPublisherOfPublication.latestForDiscovery30293a55-0e53-431f-ae8c-14ab01127be9

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IdisBa - Instituto de Investigación Sanitaria Illes Balears (Baleares)