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Two multi-fragment recombination events resulted in the β-lactam-resistant serotype 11A-ST6521 related to Spain9V-ST156 pneumococcal clone spreading in south-western Europe, 2008 to 2016

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dc.contributor.authorGonzález-Díaz, Aida
dc.contributor.authorMachado, Miguel P
dc.contributor.authorCàmara, Jordi
dc.contributor.authorYuste, Jose Enrique
dc.contributor.authorVaron, Emmanuelle
dc.contributor.authorDomenech Lucas, Mirian
dc.contributor.authorDel Grosso, María
dc.contributor.authorMarimón, José María
dc.contributor.authorCercenado, Emilia
dc.contributor.authorLarrosa, Nieves
dc.contributor.authorQuesada, María Dolores
dc.contributor.authorFontanals, Dionisia
dc.contributor.authorEl-Mniai, Assiya
dc.contributor.authorCubero, Meritxell
dc.contributor.authorCarriço, João A
dc.contributor.authorMartí, Sara
dc.contributor.authorRamirez, Mario
dc.contributor.authorArdanuy, Carmen
dc.contributor.funderCentro de Investigación Biomedica en Red - CIBER
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderGovernment of Catalonia (España)
dc.date.accessioned2020-05-06T08:31:10Z
dc.date.available2020-05-06T08:31:10Z
dc.date.issued2020-04
dc.description.abstractBackgroundThe successful pneumococcal clone Spain9V-ST156 (PMEN3) is usually associated with vaccine serotypes 9V and 14.AimOur objective was to analyse the increase of a serotype 11A variant of PMEN3 as cause of invasive pneumococcal disease (IPD) in Spain and its spread in south-western Europe.MethodsWe conducted a prospective multicentre study of adult IPD in Spain (2008-16). Furthermore, a subset of 61 penicillin-resistant serotype 11A isolates from France, Italy, Portugal and Spain were subjected to whole genome sequencing (WGS) and compared with 238 genomes from the European Nucleotide Archive (ENA).ResultsAlthough the incidence of serotype 11A in IPD was stable, a clonal shift was detected from CC62 (penicillin-susceptible) to CC156 (penicillin-resistant). By WGS, three major 11A-CC156 lineages were identified, linked to ST156 (n = 5 isolates; France, Italy and Portugal), ST166 (n = 4 isolates; France and Portugal) and ST838/6521 (n = 52 isolates; France, Portugal and Spain). Acquisition of the 11A capsule allowed to escape vaccine effect. AP200 (11A-ST62) was the donor for ST156 and ST838/6521 but not for ST166. In-depth analysis of ST838/6521 lineage showed two multi-fragment recombination events including four and seven fragments from an 11A-ST62 and an NT-ST344 representative, respectively.ConclusionThe increase in penicillin-resistant serotype 11A IPD in Spain was linked to the spread of a vaccine escape PMEN3 recombinant clone. Several recombination events were observed in PMEN3 acquiring an 11A capsule. The most successful 11A-PMEN3 lineage spreading in south-western Europe appeared after two multi-fragment recombination events with representatives of two major pneumococcal clones (11A-ST62 and NT-ST344).es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis study was supported by grants from Fondo de Investigaciones Sanitarias de la Seguridad Social (PI14/00627; PI18/00339, INT 15/0186; INT16/0117) and from Centro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES) CB06/06/0037), an initiative of the Instituto de Salud Carlos III, Madrid, Spain and ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI – ‘Fundos Europeus Estruturais e de Investimento’ from ‘Programa Operacional Regional Lisboa 2020’ and by national funds from FCT – ‘Fundação para a Ciência e a Tecnologia’. Financial support was also provided by the European Regional Development Fund (ERDF). We thank CERCA Programme /Generalitat de Catalunya for institutional support.es_ES
dc.format.number16es_ES
dc.format.volume25es_ES
dc.identifier.citationEuro Surveill. 2020 Apr;25(16):1900457.es_ES
dc.identifier.doi10.2807/1560-7917.ES.2020.25.16.1900457es_ES
dc.identifier.e-issn1560-7917es_ES
dc.identifier.issn1560-7917es_ES
dc.identifier.journalEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletines_ES
dc.identifier.pubmedID32347199es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9916
dc.language.isoenges_ES
dc.publisherEuropean Centre for Disease Prevention and Control (ECDC)es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI14/00627es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI18/00339es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/INT 15/0186es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/INT16/0117es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CB06/06/0037es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/LISBOA-01-0145-FEDER-016417es_ES
dc.relation.publisherversionhttps://doi.org/10.2807/1560-7917.ES.2020.25.16.1900457es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCC156es_ES
dc.subjectMulti-fragment recombinationes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectVaccine escapees_ES
dc.titleTwo multi-fragment recombination events resulted in the β-lactam-resistant serotype 11A-ST6521 related to Spain9V-ST156 pneumococcal clone spreading in south-western Europe, 2008 to 2016es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication8e36a2c1-2c2c-4d30-90ad-e0d92630c74f
relation.isAuthorOfPublicationfe625b79-e715-4d78-a0ee-d2b8ae255c78
relation.isAuthorOfPublication.latestForDiscovery8e36a2c1-2c2c-4d30-90ad-e0d92630c74f
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