Publication:
Ammonia Affects Astroglial Proliferation in Culture

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URI: http://hdl.handle.net/20.500.12105/6923
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0139619
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2015-09-30
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Public Library of Science (PLOS)
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Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression. A reduction in cell viability was also noted. The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.
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Ammonia Animals Astrocytes Bromodeoxyuridine Cell Count Cell Proliferation Cell Survival Cells, Cultured Chromatin DNA-Directed DNA Polymerase Gene Expression Histones Rats S Phase Cell Cycle Checkpoints
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PLoS One. 2015 Sep 30;10(9):e0139619
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Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC)
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https://doi.org/10.1371/journal.pone.0139619
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journal article