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Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells

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dc.contributor.authorSureda Gomila, Antoni
dc.contributor.authorMartorell, Miquel
dc.contributor.authorBibiloni Esteva, Maria Del Mar
dc.contributor.authorBouzas, Cristina
dc.contributor.authorGallardo-Alfaro, Laura
dc.contributor.authorMateos, David
dc.contributor.authorCapó Fiol, Xavier
dc.contributor.authorTur, Josep A
dc.contributor.authorPons, Antoni
dc.date.accessioned2024-09-13T09:13:38Z
dc.date.available2024-09-13T09:13:38Z
dc.date.issued2020-01-04
dc.description.abstractThe aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, α-linolenic, γ-linolenic, arachidonic and docosahexaenoic at 50 μM, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NFκB, TLR2, TNFα, and COX-2 gene expressions were determined. Palmitic, γ-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, α-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and α-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, γ-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, α-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and α-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds.en
dc.description.sponsorshipInstituto de Salud Carlos III through the Fondo de Investigación para la Salud (FIS), which is cofundedby the European Regional Development Fund , EU-COST Action CA16112; Grant of support to research groupsno. (Balearic Islands Gov.), IDISBA Grants (FOLIUM, PRIMUS, SYNERGIA, and LIBERI), and Fundació La MaratóTV3 (Spain) project ref. . C.B. received a Fernando Tarongí Bauzà PhD Grant. L.G.-A. receivedan FPU PhD Grant from the Spanish Ministry of Education. M.M. was funded by Vicerrectoria de Investigación yDesarrollo from University of Concepcion, Chile. The funding sponsors had no role in thedesign of the study, in the collection, analyses, or interpretation of the data; in the writing of the manuscript, or inthe decision to publish the results.es_ES
dc.format.number1es_ES
dc.format.volume12es_ES
dc.identifier.citationSureda A, Martorell M, Bibiloni MDM, Bouzas C, Gallardo-Alfaro L, Mateos D, et al. Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells. Nutrients. 2020 Jan 4;12(1).en
dc.identifier.doi10.3390/nu12010146
dc.identifier.e-issn2072-6643es_ES
dc.identifier.journalNutrientses_ES
dc.identifier.otherhttp://hdl.handle.net/20.500.13003/18237
dc.identifier.pubmedID31947975es_ES
dc.identifier.puiL2003459656
dc.identifier.scopus2-s2.0-85077572916
dc.identifier.urihttps://hdl.handle.net/20.500.12105/22939
dc.identifier.wos516825500146
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.publisherversionhttps://dx.doi.org/10.3390/nu12010146en
dc.rights.accessRightsopen accessen
dc.rights.licenseAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.decsLipopolisacáridos*
dc.subject.decsExpresión Génica*
dc.subject.decsInterleucina-6*
dc.subject.decsMediadores de Inflamación*
dc.subject.decsFN-kappa B*
dc.subject.decsSíndrome Metabólico*
dc.subject.decsFemenino*
dc.subject.decsFactor de Necrosis Tumoral alfa*
dc.subject.decsÁcidos Grasos no Esterificados*
dc.subject.decsMasculino*
dc.subject.decsPeróxido de Hidrógeno*
dc.subject.decsReceptor Toll-Like 2*
dc.subject.decsHumanos*
dc.subject.decsPersona de Mediana Edad*
dc.subject.decsLeucocitos Mononucleares*
dc.subject.decsAnciano*
dc.subject.decsAnciano de 80 o más Años*
dc.subject.decsCiclooxigenasa 2*
dc.subject.decsEstrés Oxidativo*
dc.subject.meshMetabolic Syndrome*
dc.titleEffect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cellsen
dc.typeresearch articleen
dspace.entity.typePublication
relation.isPublisherOfPublication30293a55-0e53-431f-ae8c-14ab01127be9
relation.isPublisherOfPublication.latestForDiscovery30293a55-0e53-431f-ae8c-14ab01127be9

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