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Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

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dc.contributor.authorIglesias-Caballero, Maria
dc.contributor.authorCamarero-Serrano, Sara
dc.contributor.authorVarona Fernandez, Sarai
dc.contributor.authorMas-Lloret, Vicente
dc.contributor.authorCalvo, Cristina
dc.contributor.authorGarcía, María Luz
dc.contributor.authorGarcía-Costa, Juan
dc.contributor.authorVázquez-Morón, Sonia
dc.contributor.authorMonzon-Fernandez, Sara
dc.contributor.authorCampoy, Albert
dc.contributor.authorCuesta de la Plaza, Isabel
dc.contributor.authorPozo Sanchez, Francisco
dc.contributor.authorCasas Flecha, Inmaculada
dc.contributor.funderInstituto de Salud Carlos III
dc.date.accessioned2023-12-12T09:32:34Z
dc.date.available2023-12-12T09:32:34Z
dc.date.issued2023-12
dc.description.abstractTo advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis research was partially funded by Instituto de Salud Carlos III through the Project PI18/00167, PI21CIII/00019 (MPY 439-21) and PI21/00377, and partially by UNESPA donation- “COVIDSEQUNESPA” (grant number MPY 226/22). Also, funding was received from grant MSD MISP: IISP 60255.es_ES
dc.format.number49es_ES
dc.format.volume28es_ES
dc.identifier.citationEuro Surveill. 2023 Dec;28(49).es_ES
dc.identifier.doi10.2807/1560-7917.ES.2023.28.49.2300637es_ES
dc.identifier.e-issn1560-7917es_ES
dc.identifier.journalEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletines_ES
dc.identifier.pubmedID38062945es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16776
dc.language.isoenges_ES
dc.publisherEuropean Centre for Disease Prevention and Control (ECDC)
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/PI18 - Proyectos de investigacion en salud (AES 2018). Modalidad proyectos en salud. (2018)/PI18/00167es_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III///PI21-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2021)/PI21CIII/00019es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/MPY439-21es_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III///PI21 - Proyectos de investigacion en salud (AES 2021). Modalidad proyectos de investigación en salud. (2021)/PI21/00377es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/MPY226/22es_ES
dc.relation.publisherversionhttps://doi.org/10.2807/1560-7917.ES.2023.28.49.2300637es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiología::Unidades Comunes Científico-Técnicas (UCCT)
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleGenomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequenceses_ES
dc.typeresearch articlees_ES
dc.type.hasVersionVoRes_ES
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Centro Nacional de Microbiología (CNM)
IdiPAZ - Instituto de Investigación Sanitaria Hospital La Paz (Madrid)
IDIPHIM - Instituto de Investigación Sanitaria Puerta de Hierro (Madrid)
Unidades Centrales Científico-Técnicas (UCCTs)