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Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.

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dc.contributor.authorSadaow, Lakkhana
dc.contributor.authorRodpai, Rutchanee
dc.contributor.authorSmout, Michael J
dc.contributor.authorNakajima, Rie
dc.contributor.authorBoonroumkaew, Patcharaporn
dc.contributor.authorSotillo, Javier
dc.contributor.authorTedla, Bemnet A
dc.contributor.authorLuvira, Vor
dc.contributor.authorKitkhuandee, Amnat
dc.contributor.authorPaonariang, Krisada
dc.contributor.authorSukeepaisarnjaroen, Wattana
dc.contributor.authorYamasaki, Hiroshi
dc.contributor.authorSuttiprapa, Sutas
dc.contributor.authorLaha, Thewarach
dc.contributor.authorSripa, Banchob
dc.contributor.authorde Assis, Rafael
dc.contributor.authorJain, Aarti
dc.contributor.authorIttiprasert, Wannaporn
dc.contributor.authorMann, Victoria H
dc.contributor.authorWong, Yide
dc.contributor.authorFelgner, Philip L
dc.contributor.authorMaleewong, Wanchai
dc.contributor.authorBrindley, Paul J
dc.contributor.authorLoukas, Alex
dc.contributor.authorIntapan, Pewpan M
dc.contributor.funderNational Research Council of Thailand
dc.contributor.funderKhon Kaen University (Tailandia)
dc.contributor.funderNIH - National Cancer Institute (NCI) (Estados Unidos)
dc.contributor.funderNational Health and Medical Research Council (Australia)
dc.date.accessioned2026-09-21T11:29:06Z
dc.date.available2026-09-21T11:29:06Z
dc.date.issued2025-07-01
dc.description.abstractSensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.
dc.description.peerreviewedSí
dc.description.sponsorshipThis study was supported by grants from the National Research Council of Thailand (NRCT): High-Potential Research Team Grant Program (Contract no. N42A670561 to WM, PMI, LS, RR, PB), the Office of the Ministry of Higher Education, Science, Research, and Innovation under the Reinventing University 2024 Visiting Professor Program (WM), the Research Program from Research and Graduate studies, Khon Kaen University (KKU) (grant no. RP66-7-001 to WM), and by award R01 CA164719 (TL, MJS, PMI, PLF, PJB, AL) from the National Cancer Institue, National Institues of Health (NIH), USA. AL is supported by an investigator grant from the National Health and Medical Research Council of Australia (NHMRC) (2008450).
dc.format.number1
dc.format.page5965
dc.format.volume16
dc.identifier.citationSadaow, L., Rodpai, R., Smout, M.J. et al. Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma. Nat Commun 16, 5965 (2025). https://doi.org/10.1038/s41467-025-61043-2.
dc.identifier.doi10.1038/s41467-025-61043-2
dc.identifier.journalNature Communications
dc.identifier.pubmedID40595543
dc.identifier.urihttps://hdl.handle.net/20.500.12105/27726
dc.language.isoeng
dc.publisherSpringer Nature
dc.relation.projectIDinfo:eu-repo/grantAgreement/National Research Council of Thailand//N42A670561///
dc.relation.projectIDinfo:eu-repo/grantAgreement/Khon Kaen University (KKU)//RP66-7-001///
dc.relation.projectIDinfo:eu-repo/grantAgreement/National Cancer Institute//R01 CA164719///
dc.relation.projectIDinfo:eu-repo/grantAgreement/National Health and Medical Research Council of Australia (NHMRC)//2008450///
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-025-61043-2
dc.repisalud.centroISCIII::Centro Nacional de Microbiología (CNM)
dc.repisalud.institucionISCIII
dc.rights.accessRightsopen access
dc.rights.licenseAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAnimals
dc.subject.meshAntibodies, Helminth
dc.subject.meshAntigens, Helminth
dc.subject.meshBile Duct Neoplasms
dc.subject.meshBiomarkers
dc.subject.meshCholangiocarcinoma
dc.subject.meshFasciola hepatica
dc.subject.meshFascioliasis
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunoglobulin G
dc.subject.meshMale
dc.subject.meshOpisthorchiasis
dc.subject.meshProteome
dc.titleImmunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication
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