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Flat clathrin lattices are linked to metastatic potential in colorectal cancer

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dc.contributor.authorCresens, Charlotte
dc.contributor.authorSolis-Fernandez, Guillermo
dc.contributor.authorTiwari, Astha
dc.contributor.authorNuyts, Rik
dc.contributor.authorHofkens, Johan
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorRocha, Susana F.
dc.contributor.funderResearch Foundation - Flanderses_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)es_ES
dc.date.accessioned2023-08-28T15:19:33Z
dc.date.available2023-08-28T15:19:33Z
dc.date.issued2023
dc.description.abstractClathrin assembles at the cells' plasma membrane in a multitude of clathrin-coated structures (CCSs). Among these are flat clathrin lattices (FCLs), alternative clathrin structures that have been found in specific cell types, including cancer cells. Here we show that these structures are also present in different colorectal cancer (CRC) cell lines, and that they are extremely stable with lifetimes longer than 8 h. By combining cell models representative of CRC metastasis with advanced fluorescence imaging and analysis, we discovered that the metastatic potential of CRC is associated with an aberrant membranous clathrin distribution, resulting in a higher prevalence of FCLs in cells with a higher metastatic potential. These findings suggest that clathrin organization might play an important yet unexplored role in cancer metastasis.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThe authors would like to thank colleagues from KU Leuven MIP division, especially from the group of Prof. Rocha, for their input and critical questions. We thank Fidler’s lab (MD Anderson Cancer Center) for sharing KM12 model cell lines and Dr. Zhuang for making the EYFP-CLTA plasmid available (Addgene plasmid #20921). We also thank Prof. R. Vitale (Universite´ de Lille) for guidance and feedback on the statistical analysis. This work was funded by the Research Foundation - Flanders (C.C. is recipient of a PhD fellowship for fundamental research, FWO grant number 1121221N. G.S.-F. is recipient of a predoctoral contract, FWO grant number 1193818N), and the AES-ISCIII program to R.B. (PI17CIII/00045 and PI20CIII/00019 grants partially supported by FEDER funds). J.H. acknowledges financial support from the Research Foundation Flanders (FWO grant numbers G0C1821N and ZW15 09-G0H6316N), from the Flemish Government through long-term structural funding Methusalem (CASAS2, Meth/15/04), and from the MPI as a fellow. S.R. acknowledges financial support from KU Leuven (grant numbers KA/20/026 and IDN/20/021).es_ES
dc.format.number8es_ES
dc.format.page107327es_ES
dc.format.volume26es_ES
dc.identifier.citationiScience. 2023 Jul 12;26(8):107327.es_ES
dc.identifier.doi10.1016/j.isci.2023.107327es_ES
dc.identifier.e-issn2589-0042es_ES
dc.identifier.journaliSciencees_ES
dc.identifier.pubmedID37539031es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16365
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/PI17-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2017)/PI17CIII/00045es_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/Subprograma Estatal de Generación de Conocimiento/PI20-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2020)/PI20CIII/00019es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.isci.2023.107327es_ES
dc.repisalud.centroISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC)es_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBiomechanicses_ES
dc.subjectCanceres_ES
dc.subjectCellular physiologyes_ES
dc.subjectExperimental systems for structural biologyes_ES
dc.titleFlat clathrin lattices are linked to metastatic potential in colorectal canceres_ES
dc.typeresearch articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationb08710f3-823c-4c5e-a191-699f1d89c5b1
relation.isAuthorOfPublicationdfb1f4f9-c8e8-4087-9db5-6477d4f154e4
relation.isAuthorOfPublication.latestForDiscoveryb08710f3-823c-4c5e-a191-699f1d89c5b1

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